Part XL 
MISCELLANEOUS. 
FEEDING EXPERIMENTS WITH COOKED MEAT. 
In our former work we showed that guinea pigs may be sensitized 
feeding them blood serum or meat. We know that heating blood 
serum to 100° C. for fifteen minutes is sufficient to destroy its toxic 
action. We then asked ourselves the question, W^ould the heating of 
meat prevent the sensitizing action? The following experiments indi- 
cate that heat does destroj^ this property of meat, as far as the guinea 
pig is concerned. 
In these experiments well-cooked horse meat was used. It was 
heated in the hot air sterilizer at 110° C. for thirty minutes. Two 
grams of it was fed to the animals daily from June 15 to June 30. 
Each guinea pig, therefore, received 32 gm. of the cooked meat. 
None of them showed symptoms when injected eighteen days later 
with 6 c. c. of normal horse serum. 
No. 
G. P. 
First treatment. 
Interval. 
1 
Second treatment. 
Result. 
496 
Fed 2 gm. horse meat, 
heated to 110° C. (hot 
air) 30 minutes, daily 
for 16 days. j 
do ' 
Days. 
18 
6 c. c. normal horse 
No symptoms. 
497 
18 
(roan) serum, in- 
traperitoneally. 
do 
No symptoms. 
No symptoms. 
No symptoms. 
No symptoms. 
498 
do * 
18 
do 
499 
do 
18 
do 
500 
do 
18 
do 
FEEDING EXPERIMENTS WITH RAW BEEF. 
We know that guinea pigs fed with horse serum or horse meat are 
susceptible to subsequent injections of horse serum, and we are now 
able to show, by the following series of experiments, that guinea pigs 
fed with beef are susceptible to subsequent injections of cattle serum: 
No. 
G. P. 
First treatment. 
Interval. 
Second treatment. 
j Result. 
501 
Fed 2 gm. dried beef 
Days. 
19 
6 c. c. cattle serum. 
Mild symptoms. 
502 
daily for 23 davs. 
do 
19 
intraperitoneally. 
do 
Mild symptoms. 
Severe symptons. 
503 
do 
19 
do 
( 61 ) 
