24 
Each guinea pi^ of the second series (Table Xo. 4) received the 
same mix ture of toxine and antitoxin as those of the first series. 
"We learn from this series that three guinea pigs (Xos. 7948, 7949, 
and 7950) which received 250 units of antitoxin twenty-four hours 
after infection had paralysis but recovered. In two of them (Xos. 
7949 and 7950) the paralysis came on later than in the controls, viz, 
on the nineteenth day. It is therefore plain that the antitoxin given 
twenty -four hours after infection had the power of modifying the 
paralysis and saving the life of these animals. 
TVe learn next that 250 units of antitoxin given forty-eight hours 
or more after infection totally failed to modiX' the paralysis or save 
the lives of the guinea pigs (Xos. 7951, 7952, and 7953). 
The animals which received 250 units of antitoxin dailv beginning 
twenty-four hours after infection (Xos. 7978, 7979, and 7980) devel- 
oped paralysis late and benign in type. The symptoms appeared 
on the seventeenth, twentieth, and twenty-sixth days, respectively. 
All three recovered. Here again the antitoxin undoubtedly modified 
the severity of the paralysis and saved the lives of these pigs. 
The guinea pigs which received 250 units of antitoxin daily begin- 
ning forty-eight hours after infection nicely demonstrate the import- 
ance of using the serum early. Two (Xos. 7981 and 7982) developed 
paralysis on the seventeenth and twentieth days, respectively, and 
recovered. The other one (Xo. 7983) developed paralysis on the 
fifteenth day and died despite the administration of 1,250 units of 
antitoxin. Therefore it is plain that antitoxin given forty-eight 
hours after infection and in large doses may fail to influence the 
paralysis or save life. However, when given as late as the fourth or 
fifth day after infection and in repeated doses it seemed to exert some 
favorable action, for it will be noticed that guinea pigs Xos. 7984 to 
7992 developed s^miptoms on the average several days later than the 
controls; in one case (Xo. 7986) death was delayed, and in another 
case (Xo. 7988) the animal recovered. 
