13 
Meat immersed and quickly withdrawn from a solution of 1 : 500 was 
preserved from putrefaction for several days. 
Aronson (29) found that any proportion of formaldehyde in excess 
of 1:20.000 prevented the growth of B. tvphosus. B. anthracis. and 
Staphylococcus pyogenes aureus: 1:10.000 hindered the growth, but 
1: SO. 000 seems to have no influence. 
He found that the growth of B. diphtherife was prevented by the 
application for ten seconds of a solution of formaldehyde containing 
1:250: also, that twenty seconds ex[30sure to a solution of 1:10(» 
sterilized a culture of B. diphtheria?. 
Berhoz (30) gives the quantity of formalin to 1.000 grams required 
to check the growth of the organisms and the quantity of formalin to 
1.00(> grams which did not check their growth, as follows: 
* 
Chec'ts 
growth. 
Does not 
cheek 
growth. 
Culture from — 
LeueorrlieAl disctiai^e 
B. coK communis 
B. tvphosus 
B. anthracis 
Gram. 
0.03 
03 
.05 
.05 
' Gram. 
0.026 
.02 
.04 
.04 
Putrefaction of urine and bouillon is prevented by U.(t0 gram to 
1.000 grams. 
Coagulation of milk is prevented by <1.2 gram to 1.000 grams. 
Pieces of sterilized silk inoculated with cultures of B. anthracis and 
tvphosus were immersed in a 1:1,000. 1:500, and 1:100 solution of 
formalin, then inoculated into bouillon. The bouillon became cloudy. 
Blum (31) in his ex|)eriments added 10 c. c. of a bouillon culture 
to 90 c. c. of the formaldehyde solution, making 5 per cent of the 10 
per (^ent solution [Formalin. A. ]\1. S.] or 2 per cent formaldehyde. 
B. chicken cholera. — Seven-day culture. 37° C.. no efl'ect after ten. 
seventeen, and twenty -flve minutes: but no growth occurred when 
inoculations were made next day. 
Protem capsnlatm. — Eight-day culture. After tifteen minutes the 
power to gi’ow was much influenced, as a clouding of the inoculated 
bouillon occurred only after several days. After twenty-tive minutes 
no living bacteria remained. 
Staphyloccfccus pyogenes aurem. — Twenty-four hour culture. Growth 
much retarded by tifteen and twenty-tive minutes exposure. Inocu- 
lations after thirty-five minutes remained sterile. 
B. typhi ahdorninalis. — Xot killed by exposure for five. ten. fifteen, 
twenty-five, and thirty -five minutes. 
B. anthrack. — Bouillon culture three weeks old. Ao disinfection 
after ten. fifteen, and twenty-five minutes. Growth is. however, 
slower. Bouillon inoculation next day remained sterile. Twenty-five 
minutes exposure did not aflect virulence to white mice. 
