22 
6. Formaldehyde with milk, 1:20,000, has no effect on the activity 
of the enzyme galactose in vitro. 
7. Formaldehyde and milk,'l: 2,000, prevents the development of 
the common bacteria; 1:1,560 kills them in twenty-four hours. 
Southard (17) found that 1:1,000,000 solution of formalin will pre- 
vent development of bacteria, while 1:75,000 is germicidal. A 5 per 
cent solution gives better results than 2.5 per cent carbolic acid or 
1:500 solution of corrosive sublimate. 
Levy (18) gives a critical review of literature and methods. He 
gives 269 references to literature. Following is a tabulated statement 
of the results of various investigators: 
Growth inhibited in formaldehyde dilutions. 
Author. 
Staphylo- 
coccus 
pyogenes 
aureus. 
B. typho- 
sus. 
B. an- 
thracis. 
B. coli 
commu- 
nis. 
B. chole- 
rae. 
Observations. 
Slater and Rideal 
Loew and Walter 
1:5, 000 
1:10,000 
1:20,000 
1:15, 000 
1:10,000 
1:20,000 
1:20,000 
1:20,000 
1:15, 000 
1:10, 000 
1:20, 000 
1:25, 000 
1:7,000 
1:20,000 
1:10,000 
Weakened growth at 
1:14,000. 
No certain action at 
1:8,000. 
Minimal growth. 
1:3,000. 
1:6,250 to 1:14,300 accord,- 
ing to number of germs. 
1: 10,000 “ Holzin.” 
Aronson 
Trillat and Berlioz 
1:33,000 
Schmitt 
1:20,000 
Leroy des Barres 
1:10,000 
Schild 
1:13,000 
1:7,000 
Abel 
1 
F. Blum 
1 
Pottevin 
Rosenberg 
