26 
It will be seen from Table No. 1 that in the flask to which garden 
earth was added bacterial growth ceased in the dilution of 1 to 5,000 
and molds developed on the seventh day in the dilution of 1 to 1,000. 
In the flask to which stable manure was added bacterial growth 
occurred in the dilution of 1 to 1,000 and molds on the seventh dsij in 
1 to 1,000. 
In the flask to which were added bits of hay bacterial growths 
occurred in a dilution of 1 to 4,000 and molds on the fourth day in a 
dilution of 1 to 1,000. 
It will be seen that the lowest dilution in which bacterial growth 
occurred was 1 to 4,000 and molds in 1 to 1,000. 
ANTISEPTIC POWER AGAINST PURE CULTURES. 
The above experiment having been made to determine its restrain- 
ing influence for putrefactive changes, another series was made to 
determine its antiseptic action against pure cultures of bacteria. 
For this purpose bouillon containing various proportions of 'formalin 
was distributed in sterile test tubes, and each tube was then inoculated 
with a small loopful of a forty-eight-hour agar culture of the particular 
organism, placed in the incubator at 37^ C., and observed daily for 
fifteen days. The formalin used contained 37.2 per cent formic 
aldeh}^de. 
Table No. 4. — Bouillon containing various percentages of formalin was distributed in test 
tubes, and each tube ivas inoculated ivith a small loopful of a forty-eight-hour agar cul- 
ture of Bacillus typhosus, kept under observation in the incubator at 37° C. for fifteen days. 
[+ means growth; — no growth.] 
Dilution. 
First day. 
Second day. 
Third day. 
Fourth day. 
Fifth day. 
Sixth day. 
Seventh day. 
Eighth day. 
Ninth day. 
Tenth day. 
Eleventh day. 
Twelfth day. 
Thirteenth day. 
Fourteenth day. 
Fifteenth day. 
1 to 7,000.. 
- 
- 
- 
/ + 
\ scant. 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
1 to 6,000.. 
- 
- 
- 
- 
- 
- 
J + 
\ scant. 
U 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
1 to 5,000.. 
- 
- 
- 
- 
- 
- 
1 + 
1 scant. 
}- 
+ 
+ 
+ 
+ 
-f 
+ 
+ 
1 to 4,000.. 
1 to 3,000.. 
1 to 2,000.. 
1 to 1,000.. 
1 + 
\ scant. 
+ 
+ 
+ 
+ 
+ 
+ 
+ 
1 to 500 
