43 
SUMMARY AND CONCLUSIONS. 
Formalin in the proportion of 1 to 5,000 was sufficient to restrain 
all bacterial growth in bouillon contaminated with garden earth. 
Molds developed in a dilution of 1 to 1,000. 
In a dilution of 1 to 4,000 formalin restrains bacterial growth in 
bouillon contaminated with stable manure. 
A dilution of 1 to 4,000 restrains bacterial growth in bouillon con* 
taminated with wisps of hay. Molds developed in a dilution of 1 to 
1 , 000 . 
Summary showing lowest dilution in vjhich all visible groivth of jmre cultures was restrained. 
Organism. 
Dilution in 
; which 
growth was 
restrained. 
{ Staphylococcus epidermidis albus | 1 to 4, 000 
I Staphylococcus pyogenes albus 1 to 3, 000 
I Staphylococcus pyogenes aureus 1 to 3, 000 
[ Staphylococcus pyogenes citreus 1 to 3, 000 
' Bacillus typhosus 1 to 3, 000 
Bacillus coll communis 1 to 3,000 
^■5 Bacillus dysenteric (Shiga) 1 to 4, 000 
t Bacillus pyocyaneus 1 to 3. COO 
Bacillus mallei 1 to 6, 000 
Bacillus anthracis 1 to 4, 000 
t Bacillus pestis 1 to 5, 000 
' Bacillus megaterium — 1 to 6, 000 
; Bacillus subtilis 1 to 3, 000 
« Bacillus prodigiosus 1 to 3, 000 
Bacillus acidi lactici 1 to 6, 000 
" Bacillus proteus 1 to 6, 000 
Bacillus enteritidis 1 to 3, 000 
J Bacillus diphtheric 1 to 5, 000- 
t Vibro-cholerc 1 to 6, 000 
\ ^ 
) One per cent formalin (containing 37.2 per cent aldeh 3 ^de) killed the 
vibrio of cholera in ten minutes, B. coli in ninetj^ minutes, B. diph- 
' theriae in twenty minutes, B. mallei in ten minutes, B. p 3 ^oc}^aneus in 
fort 3 " minutes, B. dysenteriae and Staphylococcus albus grew up to 
two hours, and Stapli 3 dococcus pyogenes aureus and B. typhosus were 
killed in ninet 3 ^ minutes. 
Two per cent formalin (containing 37.2 per cent aldeh 3 "de) killed 
B. coli after sixt 3 ^ minutes’ exposure, B. diphtherite after ten minutes, 
B. p 3 ’ocvaneus ten minutes, B. d 3 ^senterite sixty minutes. Staphylo- 
coccus p 3 "ogenes albus sixt 3 ^ minutes, and Staph 3 dococcus pvogenes 
aureus and B. typhosus after fort 3 ^ minutes- 
Three per cent formalin (containing 37.2 per cent aldehvde) killed 
B. coli after thirt 3 ^ minutes’ exposure, B. dysenteriae fort 3 " minutes, 
Staphylococcus pyrogenes albus thirtv minutes. Staphylococcus pyo- 
genes aureus twenty minutes, and B. typhosus after twent 3 " minutes. 
Five per cent formalin (containing 37.2 per cent aldehyde) killed 
B. coli and Staph 3 dococcus pvogenes albus after thirt 3 " minutes’ expo- 
sure, B. dysenteriae after fortv minutes. Staphylococcus pyogenes 
aureus and B. t 3 ’phosus after tvventv minutes. 
