51 
ORIGINAL EXPERniEXTS. 
In the following work I used nine different strains of typhoid bacilli. 
The results were as follows : • 
Experiment No. 1, 
B. typhosus. 
Forty-eight hours’ growth in sterilized milk. Milk immersed in constant-temper- 
ature water bath at 60 ° C. 
Milk culture. 
Temper- 
ature 
(°C.). 
Loop of milk cr 
ture in bouillon. 
“Dawson” culture; reaction, 0.216 per cent acid: 
At start 
29 
Growth. 
IJ minutes after immersion 
55 
Do. 
58 
Do. 
4 minutes after immersion 
60 
No gro’wd;h> 
5 minutes after immersion 
(«) 
Do. 
“P. I.” culture; reaction, 0.207 percent acid: 
At start 
' 29 
Growth. 
IJ minutes after immersion 
55 
Do. 
2j minutes after immersion 
58 
Do. 
4 minutes after immersion 
60 
No growth. 
5 minutes after immersion 
(“) ; 
Growth. 
‘ ‘ P. I.” culture; reaction, 0.198 per cent acid: 
At start 
29 
Growth. 
1| minutes after immersion 
55 
Do. 
2 \ minutes after immersion 
58 
' No growth. 
4 minutes after immersion 
60 
Do. 
Sminutes after immersion 
(a) 
Do. 
“McF.” culture; reaction, 0.225 per cent acid: 
At start 
29 
j Growth. 
IJ minutes after immersion r: 
55 
Do. 
2 \ minutes after immersion 
58 
Do.= 
4 minutes after immersion 
60 
No growth. 
5 minutes after immersion 
(a) 
Do. 
“Smith” culture; reaction, 0.198 per cent acid: 
At start 
29 
Growth. 
IJ minutes after immersion 
55 
Do. 
2 \ minutes after immersion 
58 
Do. 
4 minutes after immersion 
60 
No growth. 
5 minutes after immersion 
(«) 
Do. 
“Hay” culture; reaction, 0.234 per cent acid: 
At start 
29 
Growth. 
IJ minutes after immersion 
55 
Do. 
2 \ minutes after immersion 
58 
Do. 
4 minutes after immersion 
60 
No growth. 
5 minutes after immersion 
(“) 
Do. 
“Stock” culture; reaction, 0.171 per cent acid: 
At start 
29 
Growth. 
IJ minutes after immersion 
55 
Do. 
2J minutes after immersion 
58 
No growth. 
4 minutes after immersion 
60 
Do. 
5 minutes after immersion •. 
i (a) 
Do. 
a One minute at 60°. 6 Contaminated. c Delayed. 
