52 
Experiment Xo. 1 — Continued. 
Milk culture. 
Temper- 
ature 1 
(°C.). i 
Loop of milk cul- 
ture in boullion. 
"■Chicago” culture; reaction, 0.225 per cent acid: 
At start 
29 
. Growth. 
li minutes after immersion 
55 1 
Do. 
2i minutes after immersion 
58 
No growth. 
4 minutes after immersion 
60 
Do. 
5 minutes after immersion 
(a) 
Do. 
a One minute at 60°. 
Experiment Xo. 2. 
B. typhosus. 
Culture, “Hopkins;” forty-eight hours’ growth in sterilized milk. Reaction, 0.28 
per cent acid. 
The milk at 31.5° C. immersed in constant-temperature water bath at 60° C. 
Milk culture. 
Temperature (° C.). 
j Loop of 'milk cul- 
ture in bouillon. 
Number of 
colonies 
per loop 
in agar. 
At start .... 
31.5 
Growth. 
70, 600 
li minutes after immersion 
. 
do 
25, 000 
If minutes after immersion. 
14 minutes after immersion 
57 
. Growth 
10, 140 
2\ minutes after immersion 
58 - 
do 
1,780 
2| minutes after immersion 
59 
do 
121 
3f minutes after immersion 
60 
. No growth 
1 
4f minutes after immersion 
1 minute at 60 
do 
None. 
.'if minutes after immersion 
2 minutes at 60 
. .do 
None. 
.'if minutes after immersion 
3 minutes at 60 
.do.. . . 
None. 
6f minutes after immersion. 
4 minutes at 60 
do 
None. 
7f minutes after immersion 
5 minutes at 60 
do 
None. 
Experiment Xo. 3. 
B. typhosus. 
Culture, “P. I.;” forty-eight hours’ growth in sterilized milk. Reaction, 0.198 per 
cent acid. 
Milk at 29° C. immersed in constant-temperature water bath at 60° C. 
f 
Milk culture. 
Temperature C° C.) . 
Loop of milk cul- , 
ture in bouillon. 
Number of 
colonies 
per loop 
in agar. 
.\t start. 
29 
Growth i 
(a) 
24 
Lost. 
None. 
I None. 
11 minutes after immersion 
do i 
2f minutes after immersion . . 
58 
o 
fQ 
O 
r+ 
p* 
A minutes after immersinn 
fifi 
. . ..do 
5 minutes after immersion 1 minute at 60 
do 
a Inmunerable. 
