53 
Experiment No. 4. 
B. typhosus. 
Culture, “Hopkins;” twenty-four hours’ growth in sterilized milk. Reaction, 0.27 
per cent acid. 
, The milk at 27° C. immersed in constant-temperature water bath at 60° C. 
Milk culture. 
Temperature (° C.). 
I 
Loop of milk cul- 
ture in bouillon. 
Number of 
colonies 
per loop 
in agar. 
\t start 
27 
Growth 
j 
i a 40, 000 
a 40, 800 
a 40, 600 
2, 400 
360 
12 
None. 
None. 
None. 
None. 
None. 
1 minute after immersion 
50 
.. ..do 
iif minutes after immersion 
55. - 
. . . .do. . 
2\ minutes after immersion 
58 
. do 
minutes after immersion 
59 
do 
4 minutes after immersion 
60 
do 
6 minutes after immersion 
8 minutes after immersion 
2 minutes at 60 
4 minutes at 60 
No growth 
do 
10 minutes after immersion 
6 minutes at 60 
do 
12 minutes after immersion 
8 minutes at 60 
do 
14 minutes after immersion 
10 minutes at 60 ... . 
do 
a About. 
Experiment No. 5. 
B. typhosus. 
Twenty-four houi's’ growth in sterilized milk. Milk at 28° C. immersed in constant- 
temperature water bath at 60° C. 
Milk culture. 
Temperature (° C.). 
Loop of milk cul- 
ture in bouillon. 
“Stock” culture; reaction, 0.162 percent acid: 
At start 
28 
Growth. 
No growth. 
Do. 
Do. 
Do. 
Do. 
Growth. 
No growth. 
Do. 
Do. 
Do. 
Do. 
2\ minutes after immersion. . . 
58 
3 minutes after immersion. . 
59 ' .. . . 
4 minutes after immersion 
60 
5 minutes after immersion 
1 minute at 60 
6 minutes after immersion 
2 minutes at 60 
“Hay” culture; reaction, 0.126 per cent acid: 
At start 
28 
2\ minutes after immersion. 
58 
3 minutes after immersion 
59 
4 minutes after immersion 
60 > 
5 minutes after immersion 
1 minute at 60 
6 minutes after immersion 
2 minutes at 60 
