54 
Experlment No. 6. 
B. typhosus. 
Culture, “Hay;” forty-eight hoius’ growth in sterilized milk. Reaction, 0.162 per 
cent acid. 
The milk at 24° G. immersed in constant-temperature water bath at 60° C. 
Milk culture. 
Temperature (° C.). 
1 c. c. milk culture 
in bouillon. 
At start - 
24 .! 
Growth. 
1 1 minutes after immersion 
50 
Do. 
1| minutes after immersion 
55 . . 
Do. 
2j minutes after immersion 
58 
Do. 
3j minutes after immersion ... 
60 
Do. 
4j minutes after immersion . . . . 
1 minute at 60 
No growth. 
51 minutes after immersion ... . 
2 minutes at 60 
Do. 
61 minutes after immersion 
3 minutes at 60 
Do. 
71 minutes after immersion 
4 minutes at 60 
Do. 
81 minutes after immersion 
5 minutes at 60 
Do. 
i 
Experiment No. 7. 
B. typhosus. 
Culture, “Chicago;” forty-eight hom-s’ growth in sterilized milk. Reaction, 0.171 
per cent acid. 
The milk at 24° C. immersed in constant -temperature water bath at 60° C. 
Milk culture. 
Temperature (° C.). 
1 c. c. milk culture 
in bouillon. 
At start 
24 
Growth. 
11 minutes after immersion 
50 
Do. 
1| minutes after immersion 
55 
Do. 
21 minutes after immersion 
58 
Do. 
2| minutes after immersion . . . . 
60 
Do. 
3j minutes after immersion . . . 
1 minute at 60 
No growth. 
Do. 
4j minutes after immersion 
2 minutes at 60 
5j minutes after immersion 
3 minutes at 60 
Do. 
6| minutes after immersion 
4 minutes at 60 
Do. 
7f minutes after immersion 
5 minutes at 60 
Do. 
Experiment No. 8. 
B. typhosus. 
Culture, “Hopkins;” forty-eight hoiu's’ growth in sterilized milk. Reaction, 0.153 
per cent acid. 
The milk at 24° C. immersed in constant-temperature water bath at 60° C. 
Milk culture. 
Temperature (° C.). 
1 c. c. milk culture 
in bouillon. 
At start 
24 
Growth. 
11 minutes after immersion 
50 
Do. 
If minutes after immersion 
55 
Do. 
21 rninutes after immersion 
58 
Do. 
2| minutes after immersion r 
60 
Do. 
3| minutes after immersion 
1 minute at CO 
Do. 
