55 
Experiment No. 8 — Continued. 
Milk culture. 
Temperature (°C.). 
1 1 c. c. milk culture 
, in bouillon. 
4f minutes after immersion 
2 minutes at CO 
No growth. 
Do. 
Do. 
Do. 
5| minutes after immersion 
3 minutes at 60 
6 J minutes after immersion 
4 minutes at CO 
7f minutes after immersion 
5 minutes at 60 
Experiment No. 9. 
B. typhosus. 
Culture, “P. I.;” forty-eight hours’ growth in sterilized milk. Keaction, 0.153 per 
cent acid. 
The milk at 24° C. immersed in constant-temperature water bath at 60? C. 
Milk culture. 
Temperature (° C.). 
§ c. c. milk culture 
in bouillon. 
At start . 
24 
Growth. 
IJ minutes after immersion . 
50 
Do. 
1^ minutes after immersion 
55 
Do. 
2J minutes after immersion 
58 ■ 
Do. 
minutes after immersion. 
60 
No growth. 
4J minutes after immersion 
1 minute at 60 . . . . 
Do. 
minutes after immersion 
2 minutes at 60 
Do. 
6J minutes after immersion. 
3 minutes at 60 
Do. 
?5 minutes after immersion 
4 minutes at 60 
Do. 
81 minutes after immersion 
5 minutes at 60 
Do. 
Experiment No. 10. 
B. typhosus. 
Culture, “Smith;” forty-eight hours’ growth in sterilized milk. Reaction, 0.162 
per cent acid. 
The milk at 24° C. immersed in constant-temperature water bath at 60° C. 
Milk culture. 
Temperature (° C.). 
1 c. c. milk culture 
in bouillon. 
At start 
24 
Growth. 
Do. 
I Do. 
Do. 
Do. 
No growth. 
Do. 
Do. 
Do. 
Do. 
11 minutes after immersion 
50 ... 
11 minutes after immersion 
55 
2J minutes after immersion 
58 
3J minutes after immersion 
60 
4J minutes after immersion 
1 minute at 60 
5J minutes after immersion. 
2 minutes at 60 
6J minutes after immersion. 
3 minutes at 60 . . . 
7J minutes after immersion. 
4 minutes at 60 . . . 
81 minutes after immersion. 
5 minutes at 60 
