57 
is killed at a temperature of 58° C. in ten minutes. Details not 
stated. 
Sternberg 1892, quotes Loffler to the effect that the thermal 
death point of the bacillus of diphtheria is 60° C. 
ORIGINAL EXPERIMENTS. 
In my work I used Park’s bacillus Xo. 8, which is a strong toxine 
producer and Aurulent for laboratory animals. My results follow: 
Experiment Xo. 13. 
B. diphtherise. 
Culture, “Park, Xo. 8;” twenty-four hours growth in sterilized milk. Reaction of 
milk, 0.108 per cent acid. 
The milk at 32° C. immersed in constant-temperature water bath at 60° C. 
Milk culture. 
Temperature (° C.). 
Loop of milk cul- 
ture in bouillon. 
Number of 
colonies per 
loop in glyc- 
erin agar. 
At start . 
32 
Growth 
1,240 
2 niinutes after immersion 
50 
No growth- 
None. 
2 1 minutes after immersion . . 
57 
do 
None. 
3f minutes after immersion . ... 
59 
do 
None. 
4| minutes after immersion 
60 
do 
None. 
.54 minnt.p.s After immersion 
1 minute at 60 
do 
None. 
Experiment Xo. 14. 
B. diphtherix. 
Culture, “Park, No. 8;” twenty-four hours growth in sterilized milk. Reaction of 
milk, 0.108 per cent acid. 
The milk at 31.5° C. immersed in constant-temperature water bath at 60° C. 
Milk culture. 
Temperature (°C.). 
Loop of milk cul- 
ture in bouillon. 
Number of 
colonies per 
loop in glyc- 
erin agar. 
At start 
31. 5 
Growth 
1,800 
None. 
IJ minutes after immersion 
55 
No growth 
2 minutes after immersion 
57 
do 
None. 
2J minutes after immersion 
59 
do 
None. 
3 minutes after immersion 
60.. . - .. 
..do 
None. 
4 minutes after immersion 
1 minute at 60 
fio 
None. 
! 
^Sternberg: Manual of bacteriology, 1892, p. 148; also, American Public Health Association, com- 
mittee on disinfectants, p. 152. 
