58 
Experiment Xo. 15. 
B. diphtherise. 
Culture, ‘‘Park”; forty-eight hours’ growth in sterilized milk. Reaction, 0.153 
per cent acid. 
The milk at 28° C. immersed in constant-temperature water bath at 55° C. 
Milk culture. 
Temper- 
ature 
(°C.). 
Loop of milk cul- 
ture in bouillon. 
Number 
of colo- 
nies per 
loop in 
agar. 
At start 
28 
Growth 
3, 150 
1| minutes after immersion 
45 
do 
1,890 
IJ minutes after immersion 
48 
do.a 
&19 
If minutes after immersion 
50 
do.a. . . 
26 
2 minutes after immersion. 
51 
No growth 
None. 
2j minutes after immersion 
52 
do 
None. 
2^ minutes after immersion 
53 
do 
None. 
2f minutes after immersion 
54 
do 
None. 
3| minutes after immersion 
55 
do 
None. 
a Delayed. i Contaminated. 
Experiment Xo. 16. 
B. diphtheria. 
Culture, “Park, Xo. 8;” fifty-two hours’ growth in sterilized milk. 
Milk at 28° C. immersed in constant-temperature water bath at 60° C. 
Three test-tube cultures tested by planting a loop over into bouillon. 
; Temper- 
Milk culture. I ature 
! (°C.). 
At start j 28. 5 
1^ minutes after immersion j 50 
2| minutes after immersion j 55 
2| minutes after immersion 58 
4§ minutes after immersion.^ 60 
5J minutes after immersion (a) 
Loop of milk culture in bouillon. 
Reaction, 0.171 
per cent acid. 
Reaction, 0.135 
per cent acid. 
Reaction, 0.135 
per cent acid. 
Growth 
Growth 
Growth. 
No growth. 
Do. 
Do. 
Do. 
No growth 
do 
No growth 
do 
do 
.. . do 
do 
do. . 
do 
a One minute at 60°. 
