59 
EXPER^MEXT Xo. 17. 
B. diphtherise. 
Culture, “Park, No. 8;” forty-eight hours’ growth in sterilized milk. Reaction 
of milk, 0.162 per cent acid. 
The milk at 25° C. immersed in constant-temperature water bath at 55° C. 
Milk culture. 
Temperature (°C.). 
Loop of milk cul- 
ture in bouillon. 
At start 
25 
Growth. 
1§ minutes after immersion 
45 
Do. 
2 minutes after immersion . . ... 
50 
No growth. 
Do. 
2j minutes after immersion 
51 
21 minutes after immersion 
52 
Do. 
2f minutes after immersion 
53 .’ 
Do. 
3 minutes after immersion 
54 
Do. 
3f minutes after immersion 
55 
Do. 
4f minutes after immersion 
1 minute at 55 
Do. 
5f minutes after immersion 
2 minutes at 55 
Do. 
6f minutes after immersion 
3 minutes at 55 
Do. 
Experiment No. 18. 
B. diplitherix. 
Culture, “Park, No. 8;” seventy-two hours’ growth in sterilized milk. Reaction 
of milk, 0.135 per cent acid. 
The milk at 30° C. immersed in constant -temperature water bath at 55° C. 
Milk culture. 
^ Temper- About § c. c. of 
ature milk culture into 
(°C.). , bouillon. 
Loop of milk culture in 
in bullion. 
At start .'. 
1| minutes after immersion 
11 minutes after immersion 
If minutes after immersion 
2 minutes after immersion. 
minutes after immersion 
2f minutes after immersion 
4 minutes after immersion . 
5f minutes after immersion 
30 
45 
48 
50 
51 
52 
53 
54 
55 
Growth 
do 
....do 
....do 
do 
do 
do 
Growth, delayed. . 
do 
Growth. 
Do. 
Growth, delayed. 
Growth, small, delayed. 
Growth, delayed. 
Growth, small, delayed. 
No growth. 
Do. 
Do. 
This experiment demonstrates the different results obtained by carrying over 
large and small quantities of the test fluid. 
