60 
Experiment Xo. 19. 
B. diphtheriae. 
Culture, “Park;” forty-eiglit hours’ growth in sterilized milk. Reaction, 0.09 per 
cent acid. 
The milk at 24° C. immersed in constant-temperature water bath at 60° C. 
Milk culture. 
Temperature (° C.). 
§ c. c. milk cul- 
ture in bouillon. 
At start 
24 
Growth. 
11 minutes after immersiox> 
11 minutes after immersion 
1| minutes after immersion 
2i minutes after immersion 
21 minutes after immersion 
2| minutes after immersion 
4 minutes after immersion 
5 minutes after immersion 
6 minutes after immersion 
60 
1 minute at 60 
2 minutes at 60 
bo. 
bo. 
bo. 
bo. 
bo. 
Growth, small, 
bo. 
Xo growth, 
bo. 
Experiment Xo. 20. 
B. diphtheriae. 
Culture, “Park. Xo. 8;” seventy-two hours’ growth in sterilized milk. Reaction 
of milk, 0.18 per cent acid. 
The milk at 24° C. immersed in constant-temperature water bath at 55° C. 
Milk culture. 
Temperature (° C.). 
0.5 c. c. muk cul- 
ture in bouillon. 
At start 
IJ minutes after immersion 
IJ minutes after immersion 
2 minutes after immersion. 
2i minutes after immersion 
3 minutes after immersion . 
3t minutes after immersion 
4| minutes after immersion. 
5| minutes after immersion. 
6| minutes after immersion. 
24 
45 
50 
55 
57 
5S 
59 : 
60 
1 minute at 60 . 
2 minutes at 60 
Growth.. 
bo. 
Xo growth, 
bo. . 
bo. 
bo. 
bo. 
bo. 
