63 
Friedel, Kutsclier, and Meinicke," 1904, found that the cholera 
vibrio was not killed when the temperature reached 58°, but was 
dead as soon as 60° was reached. It required seven to eight minutes 
for the milk to reach 60°. 
These experiments with the cholera organism were conducted with 
and without the enrichment methods. The enrichment methods 
consist of first planting a milk culture in peptone water, incubatmg 
twenty-four hours, and then plating into litmus-lactose-agar. With 
the enrichment method it was found that cholera withstood sixty 
minutes at 50°, but onh' twenty-five minutes without the enrichment 
method. At 53° it withstood fort}^ minutes with and fifteen minutes 
without the enrichment method. 
ORIGIXAL EXPERIMENTS. 
In my work I used three different cultures with the following results: 
Experiment Xo. 23. 
B. cholerse. 
Forty-eight hours’ growth in sterilized milk. Milk at 28° C. immersed in constant- 
temperature water bath at 55° C. 
Milk culture. - 1 
i 
Temper- 
i ature 
(°C.). 
i 
Loop of milk cul- 
ture in bouillon. 
Number 
of colo- 
nies per 
loop in 
agar. 
, 
“Manila; ” reaction, 0.180 per cent acid: 
At start 
28 
Growth 
450,000 
It minutes after immersion 
45 
do 
3,750 
Ij minutes after immersion 
48 
Xo growth 
6 10 
1| minutes after immersion 
50 
do 
None. 
2 minutes after immersion 
51 
do 
None. 
2\ minutes after immersion 
52^ 
do 
None. 
2f minutes after immersion 
53 
do 
None. 
3 minutes after immersion ^ 
54 
do •. . 
None. 
3| minutes after immersion 
55 
do 
None. 
“Hamburg;” reaction, 0.229 percent acid: 
At start r 
28 
Growth 
87, 500 
1-t minutes after immersion 
45 
do 
( 144 
1§ minutes after immersion : 
48 
Xo growth 
6 4 
If minutes after immersion ^ . 
50 
do 
None. 
2 minutes after immersion 
51 
do 
None. 
2\ minutes after immersion 
52 
do 
None. 
2f minutes after immersion 
53 
do 
None. 
3 minutes after immersion 
54 
do 
None. 
3f minutes after immersion 
55 
do 
None. 
apriedel, Kutscher, and Meinicke; Die Widerstandsfahigkeit der Erreger der wichtigsten Darm- 
krankheiten gegen Erwarmen auf verschiedene Temperaturen in Milch. Klin. Jahrh., vol. 13, 1904-05, 
p. 324. 
5 Contaminated. 
318G5— Bull. 42- 
