64 
Expeiroiext Xo. 24. 
B. cJiolei'p^. 
Three days’ growth in sterilized milk. Milk at 2S° C. immersed in constant-tem- 
perature water bath at 60° C. 
Milk culture. 
Temper- 
ature 
("C.). 
Loop of milk cul- 
ture in bouillon. 
X umber 
of colo- 
nies per 
loop in 
agar. 
“Manila;” reaction, 0.219 percent acid: 
At start - 
14 minutes after immersion 
2 minutes after immersion 
3 minutes after immersion 
5^ minutes after immersion 
“Paris;” reaction, 0.1S9 per cent acid: 
At start - 
IJ minutes after immersion. 
2 minutes after immersion 
3 minutes after immersion 
54 minutes after immersion 
2S Growth («) 
.50 (Lost 
5a Xo growth 
58 do None. 
GO' do None. 
28 Growth (a 
50 No growth 
55 do 
58 do None. 
GO do None. 
o Innumerable. 
Experiment Xo. 25. 
B. cholerx. 
Culture, ‘‘Hamburg;’' seventy-two hours' growth in sterilized milk. Reaction of 
milk, 0.216 per cent acid. 
The milk at 31° C. immersed in constant-temperature- water bath at 55° C. 
Milk culture. 
Temper- 
ature 
(° C.). 
Loop of 
milk cul- 
ture in ■■ 
agar. 
At start 
1| minutes after immersion. 
1| minutes after immersion. 
If minutes after immersion. 
2 minutes after immersion. . 
2J minutes after immersion. 
24 minutes after immersion. 
3 minutes after immersion, . 
4 minutes after immersion, . 
31 
(«) 
45 
41 
4S 
None. 
50 
None. 
51 
None. 
52 
None. 
53 
None. 
54 
None. 
55 
None. 
a Innumerable. 
