65 
Experiment No. 26. 
B. cholerse. 
Culture, “Manila;” seventy-two hours’ growth in sterilized milk. Keaction of 
milk, 0.210 per cent acid. 
The milk at 31° C. immersed in constant-temy^erature water bath at 55° C. 
Milk culture. 
T emper- 
ature 
(° C.). 
Loop of 
milk cul- 
ture in 
agar. 
At start 
1 minute after immersion. . . 
ly minutes after immersion. 
1| minutes after immersion. 
2 minutes after immersion. . 
2y minutes after immersion. 
2f minutes after immersion. 
4 minutes after immersion. . 
5 minutes after immersion. . 
31 
(“) 
4-5 
6,600 
48 ' 
628 
50 
546 
.51 
0 
52 
0 
53 
0 
54 
0 
55 
0 
o Imiumerable. 
Experiment Xo. 27. 
B. cholera. 
Culture, “Manila;” twenty-four hours’ growth in sterilized milk. Reaction, 0.180 
yier cent acid. 
The milk at 24° C. immersed in constant-temperature water 1)ath at 55° C. 
Milk culture. 
Temperature (° C.). 
Number 
colonies 
per loop in 
gelatin. 
At start 
1 
24... 
(“) 
ly minutes after immersion 
45 
1,164 
2 minutes after immersion 
50 
32 
2h minutes after immersion 
52 
0 
2 J minutes after immersion 
53 
0 
3| minutes after immersion 
54 
0 
5 minutes after immersion i 
55 
0 
6 minutes after immersion i 
1 minute at 55 
0 
7 minutes after immersion 
2 minutes at 55 
0 
8 minutes after immersion 
3 minutes at 55 . . . 
0 
a Innumerable. 
