66 
Experiment Xo. 28. 
B. cholerx. 
Culture. "Manila:” tM-enty-four hours' gi*owth in sterilized milE. Reaction, 0.198 
per cent acid. 
The milk at 24° C. immersed in constant-temperature water hath at 55° C. 
Milk culture. 
Temperature (° C.). 
Number 
colonies 
per loop in 
gelatin. 
At start 
minutes after immersion 
2 minutes after immersion. 
2\ minutes after immersion. 
3 minutes after immersion . 
3| minutes after immersion 
4| minutes after immersion 
of minutes after immersion 
6| minutes after immersion 
7| minutes after immersion. 
24 (a) 
^5 (6) 
50 245 
52 205 
53 22 
54 0- 
55 0 
1 minute at 55 0 
2 minutes at 55 . . . 0 
3 minutes at 55. .. 0 
a Innumerable. Innumerable, but less. 
Experiment Xo. 29. 
B. cholera:. 
Culture. "Manila;" ttventy-f our hours’ growth in sterilized milk. Reaction. 0.180 
per cent acid. 
The milk at 24° immerse<l in constant-teihperature water bath at 55° C. 
Milk culture. 
Temperature (° C.). 
Number 
colonies 
per loop in 
gelatin. 
At start. 
It minutes after immersion. 
2 minutes after immersion. . 
21 minutes after immersion . 
2| minutes after immersion. 
31 minutes after immersion 
4| minutes after inmrersion 
5| minutes after immersion. 
6| minutes after innnersion. 
7| minutes after immersion. 
24. 
45. 
52. 
54 
55 
1 minute at 55 
2 minutes at 55. . . 
3 minutes at 55 . . . 
(«) 
1,6S0 
244 
47 
0 
0 
0 
0 
0 
0 
a Innumerable. 
