67 
Experiment Xo. 30. 
B. cholerx. 
Culture, ‘‘Manila;’’ twenty-four hours’ growth in sterilized milk. Reaction, 0.181 
per cent acid. 
The milk at 24° C. immersed in constant-temperature water hath at 55° C. 
Milk culture. 
I 
T emperature (° C.) . 
Number 
colonies 
per loop in 
gelatin. 
At start 
It minutes after immersion 
2 minutes after immersion. 
2| minutes after immersion 
3 minutes after immersion. 
3j minutes after immersion 
5j minutes after immersion 
6t minutes after immersion 
71 minutes after immersion 
81 minutes after immersion. 
45. 
50. 
52. 
53. 
54 
55 
1 minute at 55 
2 minutes at 55 . .. 
3 minutes at 55 . . . 
4, 455 
1,100 
90 
0 
0 
0 
0 
0 
a Innumerable. 
Experiment Xo. 31. 
B. cholerse. 
Culture, “Hamburg;” two days’ growth in sterilized milk. Reaction, 0.189 per 
cent acid. 
Milk immersed at 25.3° C. in constant-temperature water hath at 55° ('. 
Loop of milk 
Milk culture. , Temperature (° C.). culture in 
bouillon. 
At start 
11 minutes after immersion 
2 minutes after immersion . 
21 minutes after inmiersion 
21 minutes after immersion 
2| minutes after immersion 
31 minutes after immersion 
41 minutes after immersion 
51 minutes after immersion 
61 minutes after immersion 
71 minutes after immersion 
25.3 
45 
Do. 
50 
. ... No growth.. 
51 
Do. 
52 
Do. 
53 
Do. 
54 
Do. 
55 
Do. 
1 minute at 55 
Do. 
2 minutes at 55..:. 
Do. 
3 minutes at 55 
Do. 
