68 
Experiment No. 32. 
B. cholerse. 
Culture, “Manila;” forty-eight hours’ growth in sterilized jnilk. Reaction, 0.180 
per cent acid. 
Milk at 26.3° C. immersed in constant-temperature water hath at 55° C. 
Milk culture. 
Temperature 
("R)- 
Loop of milk 
culture in 
bouillon. 
At start . 
26. .3 
Growth. 
1^ minutes after immersion 
45 ’ 
Do.' 
1| minutes after immersion , 
50 ' 
Do. 
2\ minutes after immersion 
51 
No growth. 
2h minutes after immersion ^ 
1 52 
Do. 
2i minutes after immersion 
53 
Do. 
3j minutes after immersion . . 
54 ■' 
Do. 
4i minutes after immersion 
55 
Do. 
51 minutes after immersion J 
1 1 minute at 55 
Do. 
6j minutes after immersion 
' 2 minutes at 55 
Do. . 
7i‘ minutes after immersion 
3 minutes at 55 
Do. 
Experiment No. 33. 
B. cholera. 
Culture, “Hamlmrg;” seventy-two hours’ groM’th in sterilized milk. Reaction of 
milk, 0.216 per cent acid. 
The milk at 31° C. immersed in constant-temperature water l>ath at 55° C. 
Milk culture. 
Temper- 
ature 
(°C.). 
About 3 c. c. 
of milk culture 
into bouillon. 
At start 
31 
Growth. 
1 minute after immersion 
45 
Do. 
1^- minutes after immersion 
48 
No growth. 
1| minutes after immer-sion 
50 1 
Growth. 
If minut&s after immersion 
51 
No growth. 
2 minutes after immersion 
52 
Growth. 
2J minutes after immersion 
53 
No growth. 
3 minutes after immersion 
54 
Do. 
4| minutes after immersion 
55 
Do. 
Experiment No. 34. 
B. choJersd. 
Culture, “Manila;” seventy-turn hours’ growth in sterilized milk. Reaction of milk, 
0.210 per cent acid. 
The milk at 31° C. immersed in constant-temperature water bath at 55° C. 
Milk culture. 
Temper- 
ature 
(°C.). 
About i c. c. 
of milk cul- 
ture into 
bouillon. 
At start 
31 
Growth. 
i minute after immersion 
45 
Do. ' 
1| minutes after immersion 
48 
Do. 
If minutes after immersion 
50 
Do. 
If minutes after immersion 
51 
Do. 
2 minutes after immersion 
i 52 
Do. 
2s minutes after immersion 
i 53 ! 
Do. 
3 minutes after immersion 
i 54 ! 
(Lost.) 
4§ minutes after immersion 
55 : 
No growth. 
