69 
Experiment No. 35. 
B. cholerse. 
Culture, “Hamburg;” foity-eiglit hours’ growuh in sterilized milk. Reaction, 0.140 
per cent acid. 
The milk at 24° C. immersed in constant -temperature water bath at 60° C. 
At start 
If minutes after immersion. 
1§ minutes after immersion 
2 minutes after immersion. 
2\ minutes after immersion 
2| minutes after immersion 
minutes after immersion 
4t minutes after immersion . 
minutes after immersion 
minutes after immersion. 
Milk culture. 
! 3 c. c. milk 
Temperature (°C.). culture in 
bouillon. 
24 Growth. 
45 ; Do. 
50 Do. 
I 53 '. Do. 
; 55 Do. 
57 NogroMTh. 
59 Do. 
60 Do. 
1 minute at 60 Do. 
2 minutes at 60 1 Do. 
Experiment Xo. 36. 
B. cholerse. 
Culture, “Manila;” foriy-eight hours’ grotvth in sterilized milk. Reaction, 0.09 per 
cent acid. 
The milk at 24° C. immersed in constant -temperature tvater bath at 60° C. 
Milk culture. 
At start 
1\ minutes after immersion 
1§- minutes after immersion 
2 minutes after immersion. 
2J minutes after immersion. 
2| minutes after immersion 
3J minutes after immersion 
4h minutes after immersion 
5^ minutes after immersion 
minutes after immersion 
'Temperature (°C.).' 
i 4 c. c. milk 
culture in 
bouillon. 
24 
Growth. 
45 ' 
Do. 
50 
Do. 
53 ’ 
Do. 
55 
No growth. 
57 
Do. 
59 
Do. 
60 
Do. 
1 1 minute at 60 
Do. 
2 minutes at 60 
Do. 
Experiment Xo. 37. 
B. cholera. 
Culture, “Hamburg;” forty-eight hours’ growth in sterilized milk. Reaction, 0.189 
per cent acid. 
The milk at 26° C. immersed in constant -temperature M'ater bath at 60° C. 
Milk culture. 
Temperature (°C.). 
t c. c. milk cul- 
ture in bouillon 
At start 
It minutes after immersion 
26 
. . 52 
Growth. 
Do. 
2 minutes after immersion 
55 - 
Do. 
.5 minutes after immersion 
. ..... 60 
No growth. 
Do. 
Do. 
Do. 
6 minutes after immersion 
7 minutes after immersion 
8 minutes after immersion ' 
1 minute at 60 
2 minutes at 60 
3 minutes at 60 
