70 
Experiment Xo. 38. 
B. cholerse. 
Culture, “Manila;” forty-eight hours’ growth in sterilized milk. Reaction, 0.16 
per cent acid. 
The milk at 20° C. immersed in constant-temperature water bath at 60° C. 
Milk culture. 
Temperature (°C.). 
I c. c. milk cul- 
ture in bouillon. 
At start 
26. . 
I Growth. 
IJ minutes after immersion 
52. 
Do. 
2 minutes after immersion 
5a 
i Do. 
5 minutes after immersion • 
60 
Do. 
No growth. 
Do. 
Do. 
6 minutes after immersion 
1 minute at 60.. . 
7 minutes after imimersion 
8 minutes after immersion 
2 minutes at 60 
3 minutes at 60... . 
Experiment Xo. 39. 
B. cholerx. 
Culture, “Hamburg;” forty-eight hours’ growth in whole milk that had been ster- 
ilized by the fractional method three days. Reaction, 0.243 per cent acid. 
The milk at 21° C. immersed in constant -temperature M'ater bath aC60° C. 
Milk culture. 
j Temperature (° C). 
1 c. c. milk cul- 
ture in bouillon. 
At start 
21. 
Growth. 
IJ minutes after immersion 
50 
Do. 
li minutes after immersion 
53 
Do. 
If minutes after immersion 
55 
Do. 
2| minutes after immersion. . 
.... 57 -.1 
1 No growth. 
3 minutes after immersion 
. 59 
Do. 
minutes after immersion 
60 
Do. 
5J minutes after immersion 
1 minute at 60 
Do. 
6^- minutes after immersion ' 
2 minutes at 60 
Do. 
minutes after immersion 
i 3 minutes at 60 
Do. 
i ■ 
Experiment Xo. 40. 
B. cholerae. 
Culture, “Manila;” forty-eight hours’ growth in -whole milk that had lieen sterilized 
by the fractional method three days. Reaction, 0.234 per cent acid. 
The milk at 21° C. immersed in constant-temperature water bath at 00° C. 
Milk culture. 
1 
Temperature 
rc.;. 
1 c. c. milk 
culture in 
bouillon. 
At start 
21 
Growth. 
Do. 
Do. 
No growth. , 
Do. 
Do. 
Do. 
Do. 
Do. 
Do. 
IJ minutes after immersion 
50 
2 minutes after immersion 
53 
2 \ minutes after immersion 
55 
2f minutes after immersion 
3J minutes after immersion 
59 
5 minutes after immersion 
GO 
6 minutes after immersion 
7 minutes after immersion 
8 minutes after immersion ! 
1 
1 minute at 60 
2 minutes at 60 . . . 
3 minutes at 60. . . 
