80 
Expeeimext No. 57. 
J/. melitensis. 
Culture, seven days’ growth, in bouillon, mixed with milk that had been inoculated 
tbe pre’vdous day with same culture. Eeaction of milk, 0.081 per cent acid. 
The milk at 31° C. immersed in constant-temperature water bath at 55° C. 
At start 
1 minute after immersion . . 
minutes after i mm ersion 
If minutes after immersion 
2 minutes after immersion . 
21 minutes after immersion 
2§ minutes after immersion 
3 minutes after immersion. 
minutes after imiaersion 
ililk culture. 
1 
Temper- 
ature 
(°C.). 
About Jc.c. 
of milA cul- 
ture into , 
bouillon. : 
Loop of 
milk cul- 
ture in 
: bouillon. 
31 
Growth. . . 
Growth. 
45 
do.... 
Do. 
48 
do 
Do. 
50 
do 
Do. 
51 
do 
Do. 
52 
do.... 
Do. 
53 
do 
Do. 
54 
do 
Do. 
55 
do 
Do. 
Experiwext Xo. 58. 
M. melitensis. 
Five days’ growth in sterilized milk. Milk immersed in coAstant-temperature 
water bath at 60° C. 
1 
Milk culture. 
Temperature (° C.). 
Xmnber of colonies 
per loop in agar. 
1 
‘‘Stock’’ culture: reaction, 0.108 per cent acid: 
At start - ! 
30.. 
Innumerable. 
i minute after immersion. 
45 
bo. 
1 minute after immersion. : 
50 
Do. 
IJ minutes after immersion. | 
55 
Do. 
If minutes after immersion. : 
58 ' 
53. 
2 minutes after immersion. 
60 ■ 
0 
3 minutes after immersion. 
1 minute at 60 
0 
•“17. S. X.” culture; reaction. 0.072 per cent acid: j 
At start 
33 1 
Innumerable. 
i minute after immersion. 1 
4-5 i 
Do. 
1 minute after immersion. i 
50 1 . .: 
Do. 
IJ minutes after immersion. • 
55 
Do. 
2 minutes after immersion. ! 
58 
Do. 
24 minutes after immersion. ; ! 
60 
0 
3 J minutes after immersion. ! 
1 minute at 60 ' 
0 
XoTE.— Agar plates incubated fire days at 37° C. 
Conclusion . — ^So far as can be judged from the meager exidence at 
hand, 60° C. for twenty minutes is more than sufficient to destroy 
the infective principle of Malta fever in milk. The J/. melitensis is 
not destroyed at 55° for a short time; the great majoritv die at 58°; 
at 80° all are killed. 
