\18 
The effect of sunffght upon this toxine ^11 be seen in the follow- 
ing tables: 
THE STABILITY OF THE STANDARD TETANUS TOXINE. 
In the following experiments the toxine (Xo. A.) was placed in small 
wide-mouthed Tials, loosely stopped with a pledget of absorbent 
cotton and exposed rmder the conditions stated in each table. Table 
Xo. I. page 16, shows the stability of this toxine when kept under 
special conditions and serves as a control for tables 5 to 8. 
Table No. 5. — Influence of sunlight. 
[ML D = . 000,006 gram.] 
Guinea 
pig Xo. 
W eight 
in grams. 
Date. 
Time of 
death. 
Amount tox- 
ine subcuta- 
neously. 
Remarks. 
Days. Hours. 
Gram. 
156 
375 
June 23.1906 
6 
18 
0. 000. 004 
1 
157 
3S5 
do 
5 
19 
.000.005 
Exposed 54 minutes to direct sunlight 
158 
400 
do 
4 9 
.000.006 
1 and 60 hours to bright light in front 
of window. 
159 
440 
do 
4 6 
.000. 007 
160 
4S0 
do 
6 8 
.OOO.OftS 
J 
171 
320 
June 26, 1906 
4 
2 
.000.006 
] Exposed .54 minutes, again 66 min- 
^ utes to direct sunlight; and oj days 
] bright light in front of window. 
172 
320 
do 
4 
0 
.000. 007 
173 
3-20 
do 
2 
20 
.000,008 
193 
310 
July 3.1906 
(“) 
.000.006 
1 Same toxine exposed 4 times, about 1 
194 
350 
Ido. 
(‘') 
.000.007,2 
^ hour each, bright sunlight and in 
195 
370 
do 
(a) 
. 000. 008. 1 
1 front of window, 13 days. 
190 
191 
192 
370 
375 
375 
do 
do 
do 
8 
6 
5 
21 
20 
23 
.000.006 
.000. 007.2 
. 000. 008. 1 
1 Control. Same toxine kept in vacuum 
j" desiccator in dark at 15° C. 
a Symptoms. 
It is evident from the above that two exposures of one hour each 
to bright sunffght, and five and one-half days to bright fight before 
a ^vindow with a southern outlook had no appreciable effect on the 
dried toxine. However, four exposures of one hour each upon 
different days to sunlight and' thirteen days in bright fight slightly 
diminished the virulence of the poison. 
Table Xo. 6. — Influence of tem perature 37® C. 
[MLD =0.000,006 gram.] 
Guinea 
pig Xo. 
W eight 
in grams. 
Date. 
Time of 
death. 
Amount toxine 
subcutane- 
ously. 
Remarks. 
Days. Hours. 
Gram. 
• 
161 
375 
June 23,1906 
5 
20 
0. 000, 004 
60 hours at 37° C. 
162 
410 
do 
6 
1 
. 000, 065 
Do. 
163 
480 
do 
5 
15 
.000.006 
Do. 
164 
480 
do 
6 
15 
.000. 007 
Do. 
165 
490 
do 
6 
6 
.000.008 
Do. 
174 
3-20 
June 26, 1906 
5 
1 
.000,006 
5^ days at 37° C 
175 
320 
do 
5 
1 
.000,007 
Do. 
176 
375 
do 
4 
4 
.000,008 
Do. 
