Table No. 6. — Influence of temperature ^1° C. — Continued. 
Guinea, 
pig No. 
Weight 
in grams. 
Date. 
Time of 
death. 
Amount toxine 
subcutane- 
ously. 
Remarks. 
196 
300 
■July 3, 1906 
Days. Hours. 
(C 
Gram. 
0. 000, 006 
13 days at 37° C. 
197 
. ..do 
(a) 
(U 
(C 
. 000, 007 
. 000, 008, 1 
. 000, 006 
Do. 
198. ... 
360 
..do 
Do. 
217 
300 
Sept. 25, 1906 
97 days at 37° C. 
218 
325 
do 
(6) - 
(C 
(C 
2 14 
. 000, 007, 2 
Do. 
219 
330 
do 
. 000, 009 
Do. 
220 
340 
do 
. 000, 009, 9 
. 000, 012 
Do. 
221c .... 
340 
do 
Do.c 
222. ... 
350 
do. 
(C 
(C 
(C 
3 14 
.000, 012,9 
. 000, 014, 1 
. 000, 015 
Do. 
223 
360 
do 
Do. 
224 
390 
do 
1 Do. 
225 
350 
Nov. 28,1906 
.000,004,2 
1 Control; toxine kept imder vacuum at 
226 
350 
. . .do 
4 4 
. 000, 005, 1 
15° C. 
Do. 
227 
350 
.do 
4 11 
. 000, 006 
Do. 
228 
350 
do 
4 0 
. 000, 006 
1 .000,007,2 
. 000, 008, 1 
Do. 
229 
350 
do 
3 16 
Do. 
230 
350 
do 
3 19 
Do. 
a Symptoms. 
b Slight symptoms. 
c No tetanic symptoms; died of pneumonia. 
d Marked symptoms. 
An exposure of five and one-half days at 37° C. in the incubator 
had no apparent effect upon the toxine. After ninety-seven days 
under the same conditions the poison had lost some of its toxicity. 
Table No. 7. — Influence of room temperature, diffuse light, etc. 
[MLD = .000,006 gram.] 
Guinea 
pig No. 
1 Weight 
:in grams. 
Date. 
Time of 
death. 
Amount tox- 
! ine subcuta- 
neously. 
Remarks. 
Bays. Hours. 
Gram. 
231 
Nov. .30,1906 
(a) 
0. OOOj 005 
232 
do .■ . 
11 13 
i . 000, 006 
233 
do 
6 18 
1 . 000, 007 
163 days in open vial, dark closet, room 
234 
do 
6 14 
1 . 000, 008 
■ temperature (June 20 to Nov. 30, 
235..... 
do 
6 15 
j . 000, 009 
1906). 
236 
do.. . 
6 23 
. 000, 01 
577 
320 
June 18,1907 
(&) 
1 . 000, 01 
578 
370 
do 
(c) 
. 000, 02 
579 
370 
do 
(<^) 
. 000, 04 
363 days in open vial, dark closet, room 
580 
37.5 
do 
5 12 
. 000, 06 
temperature (June 20, 1906, to June 
581 
385 
do 
4 2 
. 000, 08 
18, 1907). 
58£...:. 
400 
do 
3 21 
. 000, 1 
a Slight symptoms. 
b No symptoms, 
c Symptoms. 
