33 
Vaillard and Vincent,® 1890, had already that the contrac- 
tions in a particular innervation of the area disappeared when such 
an area was cut off by section of the cor'd. 
Knorr,^ 1897, as a result of liis work, concluded that: 
Pure tetanus poison has a strong affinity for some unknown substance in certain of 
the body cells. The amount of this affinity is as the degree of susceptibility of ani- 
mals (that have not previously been treated) for the poison. 
The antitoxin has the same property of combining with the toxine as has the above- 
mentioned unknown substance. 
In the culture media of tetanus bacilli there are also sometimes found bodies that 
have an affinity for the poison. 
The combination of the poison with all of these substances is slow. The rapidity 
depends upon the degree of the affinity and the concentration, and other conditions 
surrounding the poison. 
For ever\" animal species the dose most favorable for the production of antitoxin 
seems to be slightly less than that necessaiy to make the animal sick. Doses that are 
much smaller are not effective. Doses that are considerably higher interfere with the 
production of antitoxin. The production of antitoxin begins very soon after the 
inoculation of a favorable amount of toxine. 
The antitoxin is a product of the body and appears to be a specific substance of the 
cells. 
The tetanus toxine combines chemically, according to Milclmer,^ 
1898, with the antitoxic group of the central nervous system in the 
test tube, without the help of the living organism. This indicates, 
according to Ehrlich’s theory, that there is also a chemical union 
between the toxine and the nervous system in the living body. ' 
Marie, 1898, showed that the toxine injected into animals remains 
a variable time in the blood; this time passed, the inoculation of the 
organs and glandular secretions is negative. MTien injected under 
the sldn, a part of the toxine is taken up by the nerve fibers. He also 
found that the brain of rabbits neutralizes tetanus toxine. The cells 
of the central ganglions are much less active than those from the 
cerebral surface. 
For the purpose of testing the action of cord and brain of normal 
animals on tetanus toxine, TTassermann and Takald,^ 1898, used a 
solution of tetanus toxine, containing as a preservative an equal 
amount of glycerin, and of such strength that 0.001 c. c. was a fatal 
dose to a mouse weighing 15 grams vdtliin three days after inoculation. 
o Vaillard and ATncent: Sur. le poison tetanique. La sem. mod., vol. 10, 1890, pp. 
425-426. 
^ Knorr, A. ; Die Entsteliung de Tetanusantitoxins im Theirkoi-per und seine 
Beziehung zum Tetanusgift. Fortschr. d. Med., vol. 15, 1897, pp. 657-669. 
cMilchner, Richard: Xachweiss der chemischen Bindung von Tetanusgift druch 
Xervensubstanz. Berk klin. Woch., vol. 35, 1898. pp. 369-371. 
Marie, A.: Recherches sur les la toxine tetanique. Ann. Inst. Pasteur, vol. 11, 
1897, p. 591; vol. 12. 1898. p. 91. 
« tVassermann. A., and Takaki, T.: Deber tetanusantitoxische Eigenschaften des 
normalen Centralnervensystems. Berk klin. IVocli.. vol. 35, 1898, pp. 5-6. 
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