34 
They ixiixed a single, double, triple, ten times fatal dose, etc., of 
the tetanus toxine with 1 c. c. of emulsion of normal cord or brain in 
a flask and injected this subcutaneously in the dorsal region. As 
controls, equal amounts of emulsion of liver, kidney, spleen, marrow, 
or 1 c. c. serum of same animal were mixed with the specified quanti- 
ties of toxine and likewise subcutaneously injected into mice, and as a 
further control the tetanus toxine alone was injected. In all their 
experiments thus carried out they could, vdthout exception, demon- 
strate that normal brain or cord always had an antitoxic action, 
while no other tested organ showed tliis action. 
The antitoxic power of the brain of normal guinea pigs is so decided 
that 1 c. c. of brain emulsion will neutralize ten times the lethal dose. 
A definite retarding influence is exerted by the emulsion on a 60-times 
lethal dose. The cord is definite, though feebler, in its influence; 
its emulsion vdll neutralize three times the fatal dose. 
They were able to immunize against three and five times the fatal 
dose by an injection of emulsion of brain twenty-four hours before. 
They could even save a mouse that had received a fatal dose of toxine 
by the injection of the emulsion four to six hours after. The tetanus 
toxine-binding power of the central nervous sj^stem acts not only by 
direct mixing in vitro, but alsoiin the living organism. Their exper- 
iments lead them to belieAm that the fixing power of the central 
nervous system is due to a propert}^ of the cell and not to a water- 
soluble substance. 
Metchnikoff,® 1898, agrees with Wassermann and Takald that the 
brain and cord of guinea pigs neutralize tetanus toxine. From his 
own experiments he concludes that the antitetanic action of the 
nervous centers is a property of mammals. The nervous centers of 
the fowl are much less efficacious, the tortoise only very feebly so, 
and the frog not at all. 
Roux and Borrel,^ 1898, find that animals are much more sensitive 
to intracerebral than to subcutaneous injections of toxine. Animals 
highly resistant to injections under the skin or in the veins are sensi- 
tive to injections into the brain. The nerve cells are still sensitive 
to the toxine. 
Danysz,^^ 1898, showed that: 
The cerebral substance of guinea pigs fixes a variable amount of tetanus toxine 
according to the medium used. 
The quantities of toxine fixed by the cerebral substance are much greater when the 
liquid itself in which the emulsion has been made, exercises the least energetic modify- 
® Metchnikoff, E.; Sur Tinlluence de Torganisme sur les toxine. Ann. Inst. 
Pasteur, vol. 12, 1898, p. 81. 
^ Roux, E., and Borrel, A.: Tetanos cerebral et immunite contre le tetanos. Ann. 
Inst. Pasteur, vol. 12, 1898, p. 225. 
c Danysz; Contribution a Petude de la toxine tetanique. Ann. Inst. Pasteur, vol. 
13, 1898,^ pp. 156-168. 
/ 
