46 
Kind of serum and date 
of test. 
Result. 1 
li Kind of serum and date 
of test. 
Result. 
Standard serum — ConCd. 
Serum. Xo. y — Cont’d. 
Mouse?; poison, 1.4c. c.; 
Mouse 15; poison. 1.4c. c.; 
yellow right hind foot: 
red right hind foot; 
Fehruarr 2, 1905 
Marked symptoms. 
Fehruarr 2, 1905 
Marked symptoms. 
February 3, 1905 
Very serere symp- 
February 3, 1905 
Very serere symp- 
toms. 
toms. 
Fehruarr 4, 1905 
Tetany. 
Fehruarr 4. 1905 
Tetany. 
Fehruarr 5, 1905 
Dead, 4. 
Fehruarr 5, 1905 
Dead, 4. 
Mouses; poison, 1.5c. c.; 
Mouse 16: poison, 1.5c. c.; 
yellow left hind foot: 
red left hind foot : 
Fehruarr 2, 1905 
Very serere symp- 
Fehruarr 2, 1905 
Very serere symp- 
toms. 
toms. 
Fehruarr 3, 1905 
Tetanus. 
Fehruarr 3, 1905 
Tetanus. 
Fehruarr 4, 1905 
Dead, 3. 
Fehruars' 4,1905 
Dead, 3. 
In the above illustrated case the two series of animals reacted 
precisely the same. It therefore shows that the antitoxin tested 
was in reality six times the normal strength. 
It is evident, however, that the standardization of tetanus anti- 
toxin by the German method is much more complicated than the 
testing of diphtheria antitoxin.® 
The follovung preparations of tetanus antitoxin are foimd upon 
the German market G904) ; 
1. V. Belrring's tetanus curative serum, made by Professor v. 
Belming, officially tested by Professor Elmlich at the Frankfurt 
Institut for experiment elle Therapie. It is sold by the Marbourg 
firm of Doctor Siebert and Doctor Ziegenbein. The preparation 
is sold in two amormts, the one contaming 100 immimity imits (the 
curative dose) and the other 20 immimity imits (for prophylactic 
use). Small vials of dried antitoxin, containing 20 immimity 
imits, for dusting woimds, are also sold. 
2. Tizzoni’s tetanus serum, made by Tizzoni and Cattani. Tliis 
is sold by the firm of Merck, in Darmstadt. The preparation is 
sold in original vials of 5 grams each (normal dose) = 5,000,000 
immunity units. 
THE ITALIAN METHOD OF STANDARDIZING TETANUS ANTITOXIN. 
The Tizzoni preparation is now sold by Merck, at Darmstadt, 
and is said to contain in every cubic centimeter 80,000 immunity 
imits (I. U.): in other words, the antitoxin amount which vrill neu- 
tralize 80,000 toxine units. The toxine unit, according to this 
reckoning, is that amoimt of toxine wfiich will kill a rabbit weiglnng 
1 kilogram in from four to five days. The dried antitoxin is 
usually furnished, 0.1 of a gram of wliich represents 1 c. c. of serum. 
a V. Lingelsheim: T mrrm m'tat bei Tetanus. Kolle & Wassermann’s Handbuch^ 
vol. 4, part 2. 
