14 
THE EFFECT OF BLOOD SERUM AND BRAIN OF SENSITIZED 
GUINEA PIGS UPON THE SENSITIZING SUBSTANCES. 
Normal horse serum was mixed with an equal volume of the blood 
serum of a sensitive guinea pig and allowed to stand twenty-four 
hours at room temperature before the mixture was injected into a 
normal guinea pig. 
Normal horse serum was also mixed with the brain substance of a 
sensitive guinea pig, well rubbed up in a mortar, and allowed to stand 
at room temperature twenty-four hours. The extract, strained 
through gauze, was injected subcutaneously into normal guinea pigs. 
After a proper period of incubation these animals were tested and 
it was found that the anaphylactic phenomenon was in no wise 
modified by these procedures. 
Table No. 9. 
G. P. 
No. 
First injection. 
Inter- 
val in 
days. 
Second injection. 
Result. 
1406 
1 c. c. mixture of serum of G. P.9558 
19 
0.2 c. c. nonnal horse (Ted- 
Severe symptoms. 
+normal horse (roan) serum, 
equal parts, subcutaneously. 
dy) serum into brain. 
1407 
do . . . . 
19 
do 
Severe symptoms. 
Severe sjTnptoms. 
1405 
1 c. c. mixture of brain of G. P. 
19 
do 
9558+normal horse (roan) senmi, 
equal parts, subcutaneously. 
1408 
do 
19 
. ..do 
Severe symptoms. 
Guinea pig No. 9558 received a subcutaneous injection of .25 c. c. 
of diphtheria toxine No. 7 + 1/340 c. c. antitoxic serum (Welc. 839) 
61 days before being bled. 
