17 
Table No. 10. — The effect of heat upon the toxic principle — Continued. 
G.P. 
No. 
First injection. 
Inter- 
val in 
days. 
1 
Second injection. 
I 
Result. 
9260 
0.23 c. c. toxine No. 7+1/700 
42 
Heated to 100° C. 1 hour. 
10 c. c. same dilution (=2.5 c. c. 
No symptoms. 
9218 
c. c. antitoxic horse serum 
(Ldrl.38B). 
0.23 c. c. toxine No. 7+1/1100 
42 
serum) intraperitoneally. 
10 c. c. same dilution (=2.5 c. c. 
No sj-mptoms. 
9215 
c. c. antitoxic horse serum 
(Ldrl.21C). 
0.23 c. c. toxine No. 7+1/1080 
42 
serum) subcutaneously. 
0.25 c. c. same dilution (=2.5 c. c. 
No symptoms. 
9210 
c. c. antitoxic horse serum 
(Ldrl.21). 
0.23 c. c. toxine No. 7+1/700 
42 
serum) into brain. 
do 
No symptoms. 
9343 
c. c. antitoxic horse serum 
(Ldrl. 19B). 
0.23 c. c. toxine No. 7+1/600 
96 
20 c. c. same dilution (=2.5 c. c. 
No sjTnptoms. 
i 
9344 
c. c. antitoxic horse serum 
(Ldrl. 16F). 
0.23 c. c. toxine No. 7+1/700 
96 
serum) intraperitoneally. 
Next day 6 c. c. rmheated 
normal horse (Frank) serum 
intraperitoneally. 
20 c. c. dilution heated 
Severe symptoms. 
No symptoms. 
c. c. antitoxic horse serum 
(Ldrl. 16F). 
Next day 6 c. c. unheated 
(Frank) serum. 
Severe symptoms. 
DOES HORSE SERUM DECREASE IN TOXICITY WITH AGE? 
Besredka® states that freshly drawn horse serum is more toxic 
than the same serum thirty days old. Our o\\m results are not in 
harmony with this conclusion, as may be seen from the following 
series. 
Table No. 11. — Effect of age upon toxicity of horse serum. 
G.P. 
No. 
First injection. 
Inter- 
val in 
days. I 
1 
Second injection. 
j Result. 
i 
Serum 2 days old. 
8895 
0.245 c. c. toxine No. 42+1/180 
c. c. antitoxic horse serum 
(Cutter) subcutaneously. 
67 
6 c. c. normal horse (roan) se- 
rum subcutaneously. 
Dead in 60 minutes. 
8897 
0.254 c. c. toxine No. 42+1/250 
c. c. antitoxic horse serum 
(Cutter) . 
67 i 
do 
Dead in 14 hours. 
8871 
0.245 c. c. toxine No. 42+1/500 
c. c. antitoxic horse serum 
(Ldrl. 19C). 
67 
Dead in 2 hours. 
8896 
0.245 c. c. toxine No. 42+1/200 
c. c. antitoxic horse serum 
(Cutter) . 
67 
Very severe symp- 
toms. 
8858 
0.245 c. c. toxine No. 42+1/300 
c. c. antitoxic horse serum 
(Hubbert) . 
67 
do 
Dead in 50 minutes. 
a Ann. de I’lnst. Pasteur, vol. 21, 1907, p. 777. 
45319—08 3 
