20 
and ^’aiighan has found evidence that the toxicity of the proteins 
depends upon these same gronps. 
Fleischmann® also found that tryptic digestion destroys this char- 
acteristic species specihcity. 
TTe made several tests to determine the effect of iodine upon the 
toxic action of horse serum, and it so turned out in the preliminary 
experiments that the symptoms appeared to be profotmdly modified ^ 
in the sense that they were either delayed or inhibited. We there- 
fore tested a large niunber of guinea pigs to determine this point, 
but fotmd that so far as the toxicity of horse serum was concerned 
at the second injection it is not appreciably modified by the iodine. 
In the following series the iodine was added to horse serum in the 
proportion of 1.-5 gm. per 25 c. c. of serum and 3 gm. potassimn 
iodide to aid solution. 
Txble yo. 13. — Iod\ji€ added to serum. 
G.P. 
Xo. 
First tQjectioii. 
Inter- 
val in 
days. 
Secc>nd injection. 
Kesnlt 
0.23 c. c. toxine Xo. 7— 1 250 c.c. 
anritoxie horse seram i P. I. 
' Bur.; . 
42 
6 c. c. normal horse i F rank ; se- 
rum and iodine snbcntane- 
onsly. 1 
Bead in .50 minutes. 
02S c. c. toxine Xo. 7-M 210 c. c. 
antitoxic horse senim . Swiss 
USA . 
do. --I 
; Bead in minutes. 
0.23 c. c. toxme Xo. 7—1 3S0 c. c. 
antitoxic horse senim (MuL 
i539f. 
42 
i 
do 
Severe symptoms. 
9S32A 
0-‘23 c. c. toxine X'o. 7—1 4S1 c. c. 
antitoxic horse serum . ilul. 
2539; . 
j.-> 
1 
do : 
1 
SU^t symptoms. 
O-’XitM c. c. tetanus toxine 
A-F0.CO75 c. c. antitetanic se- 
rum 1 Stan-ianl; . 
42 
do , 
Mild symptoms. 
?7TS 
0.23 c. c. toxine Xo. 7-i-l 320 c. c. 
antitoxic horse serum f Stm. 
L^51>. 
53 
6 c. c. normal horse semm » Tei- 
dy ' and iodine subcutane- 
ously. 
Bead in 65 minutes. 
9713 
0.23 c. c. toxine Xo. 7—1 4>y) c. c. 
antitoxic horse serum ! Scher. 
II;. 
66 
do 
Slight symptoms. 
9743 
0.^3 c.c. toxine Xo. 7—1 170 c.c. 
antitoxic horse serum ( Stm. 
1110} . 
53 
do - 
1 
Slight symptoms. 
91S2 
Oi-23 c. c. toxine Xo. 7— l,14<j c. c. 
antitoxic horse serum i Mul. 
2352) . 
lOB 
6 c. c. normal horse t roam se- 
rum and iodine intraperrto- 
neally. 
Bead in 30 minutes. 
9157 
0.23 c. c. toxine Xo. 7 4-1 320 c. c. 
antitoxic horse serum (Mul. 
23^;. 
108 
do 
Bead in 45 minutes. 
91S9 
0-23 c. c. toxine Xo. 7—1 .300 c. c. 
antitoxic horse serum Mul. 
2433) . 
108 
Bead in 15 minutes. 
97S3 
0.23 c. c. toxine Xo. 7—1 300 c. c. 
antitoxic horse serum Stm. 
mo>. 
aZeit.i. 
.53 6 c. c. normal horse . roan se- j 
rum and iodine subcutane- 
ously. 1 
Min. Med., voL S*. 1906, p. 515. 
Mild symptoms. 
