PART 4.— SPECIFICITY. 
SPECIFIC NATURE OF “ANAPHYLACTIN.” 
In a previous publication® on the specific nature of anaphylaxis 
we demonstrated that guinea pigs may be in a condition of anaphy- 
laxis to three protein substances at the same time. Hypersuscepti- 
bility to each protein is manifested by a second injection of the corre- 
sponding protein. The three reactions may be obtained in the same 
guinea pig within a short space of time and are as distinct and specific 
as three separate infectious diseases. 
We now bring forward experimental data proving that the sub- 
stance in the blood serum of sensitized guinea pigs, known as anaphy- 
lactin, is also specific in the same sense. In the follovdng table it will 
be seen that by transferring the blood serum of guinea pigs sensitized 
to horse serum, egg white, and milk three separate and distinct reac- 
tions were obtained in the guinea pig into which this serum was trans- 
ferred: 
Table No. 19. — The specific nature of anaphylactin. 
G. P. 
No. 
First injection. 
Inter- 
val in 
days. 
Second injection. 
Result, 
9619 
023c.c.toxineNo 7+l/600c.c. i 
taneously . 56 day s later 1 c . c 
1 c. c. egg white subcutaneous! 
centrifuged, and serum used t( 
intitoxic horse serum (Alex. A249) subcu- 
. cow’s milk subcutaneously. 1 day later 
[y. 22 days later bled; blood defib rinated 
) inject G. P. No. 9619 A. 
9619A 
7c.c.serum of G. P.9619 sub- I 
cutaneously. 
2 
6 c.c. milk intraperitoneally 
2 days later 0.2 c. c. normal horse 
(Teddy) serum into brain. 
Mild symptoms. 
Dead in 5 minutes. 
9620 
Same treatment as G. P. No. 9619. 
9620A 
Sc.c.serum of G. P.9620 sub- 
cutaneously. 
2 
6 c.c. milk intraperitoneally 
2 days later 3 c. c. egg white in- 
traperitoneally. 
2 hours later 0.2 c. c. normal 
horse (Teddy) serum into 
brain. 
Slight symptoms. 
Mild symptoms. 
Dead in 4 minutes. 
9621 
0.23 c. c. toxine No. 7-l-l/o80 c.c. antitoxic horse serum (Alex. A249) . Sub- 
sequent treatment same as G. P. 9619. 
9621A 
6.5 c. c. serum of G. P. 9621 sub- 
cutaneously. 
2 
6 c. c. milk intraperitoneally 
2 days later 6 c. c. saturated so- 
lution egg white intraperito- 
neally. 
2 hours later 0.2 c. c. normal 
horse (Teddy) serum into 
brain. 
Shght symptoms. 
Marked symptoms. 
Very severe symp- 
toms. 
9624 
0. 23 c. c. toxine No. 7-t- 1/560 c.c. antitoxic horse serum (Alex.A249). Sub- 
sequent treatment same as G. P. 9619. 
9624A 
7 c.c. serum of G. P.9624 sub- 
cutaneously. 
2 
6 c. c. milk intraperitoneally 
2 day s later 0 2 c . c . normal horse 
(Teddy) serum into brain. 
Slight symptoms. 
Dead in 5 minutes. 
aRosenau, M. J., and. Anderson, John F.: The specific nature of anaphylaxis. Jour. Infec. Dis., 
vol. 4, No. 4, Nov., 1907, pp. 552-557. 
45319—08 4 
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