32 
Table Xo. 31 . — Anaphylactin during the period of intubation — Continued. 
G. P. 
No. 
First mjection. 
Inter- 
val in 
days. 
Second injection. 
Result. 
1347A 
3 c. c. serum G. P. 1347, whicti was 
bled 5 days after injection of 
0.01 c. c. normal horse serum. 
1 
6 c. c. normal horse serum 
intraperitoneally. 
No symptoms. 
1.347B 
do 
1 
do 
No symptoms 
134SA 
5 c. c. serum G, P. 1.34S, which was 
bled 6 days after mjection of 
0.01 c. c. normal horse serum. 
1 
do 
No symptoms. 
134SB 
do 
1 
do 
No symptoms. 
1349A 
4 c. c. serum G. P. 1.349, which was 
bled 7 days after injection of 
0.01 c. c. normal horse serum. 
1 
do 
No symptoms. 
1.3oOA 
a c. c. serum G. P. 13a0, which was 
bled 8 days after mjection of 
0.01 c. c. normal horse serum. 
1 
do 
No symptoms. 
13olA 
6 c. c. serum G. P. 1351, which was 
bled 9 days after injection of 
0.01 c. c. normal horse serum. 
1 
do 
No symptoms. 
1353A 
S c. e. serum G. P. 1353, which was 
bled 10 days after injection of 
0.01 c. c. normal horse serum. 
2 
do 
Marked symptoms. 
13.S4A 
6.5 e. c. serum G. P. 1354, which 
was bled 11 days after injection 
of 0.01 c. c. normal horse serum. ; 
1 
do 
Slight symptoms. 
13o5A 
65 c. c. serum G. P. 1355, which 1 
was bled 13 days after injection 
of OBI c. c. normal horse serum. 
1 
do 
Slight symptoms. 
1355B 
1 c. c. same serum 
2 
do 
Marked symptoms. 
13o6A 
3 e. c. serum G. P. 1356, which was 
bled 13 days after injection of 
0.01 c. c. normal horse serum. 
1 
do 
No symptoms. 
1356B 
2.5 same serum 
2 
do 
Mild symptoms. 
1357A 
3 c. c. serum G. P. 1357, which was 
bled 14 . days after injection of 
0.01 c. c. normal horse serum. 
1 
do 
Mild symptoms. 
1357B 
2.5 same serum 
3 
do 
Severe symptoms. 
The first indication of anaphylactin apneared in the above series 
on the tenth day, that is, just about the time necessary to render 
guinea pigs sensitive. It is also evident that forty-eight hours is a 
better interval than twenty-four for the purpose of demonstrating 
the presence of anaphylactin in guinea-pig serum. 
Our interest in tliis subject led us to sensitize another series of 
guinea pigs with 1/100 c. c. normal horse serum subcutaneously, but 
to test for the presence of anaphylactin by injections into the brain. 
