34 
In the above series the anaphylactin appeared in the blood of 
sensitized guinea pigs on the ninth day. 
ANAPHYLACTIN IN MAN AND OTHER ANIMALS. 
The presence of anaphylactin having been demonstrated in the 
blood serum of sensitized guinea pigs, it is interesting to know whether 
man and other animals that have received a previous injection of 
horse serum also contain a similar substance. 
So far as may be judged from the following limited experiments 
upon the subject, we have been unable to demonstrate a similar 
property in the blood serum of man, the monkey, rabbit, and the 
cat when tested upon guinea pigs. 
Table No. 33. — Anaphylactin in man, monhey, rahhit, and cat. 
G. P. 
No. 
First injection. 
Inter- 
val in 
days. 
Second injection. 
Result. 
Man. 
1310 
Ic.c. normal human serum (CWC) 
subcutaneously. 
6 c. c. normal horse (roan) 
serum intraperitoneally. 
Slight symptoms. 
280 
5 c. c. human serum (LLL). 7 
years prior this man had been 
injected with 12 c. c. antitoxic 
horse serum. 
1! 
do 
No symptoms. 
1311 1 
0.4 c. c. human serum (JWT). 1 
year prior this man had been 
injected with 5,000 units anti- 
toxic horse serum. 
34 
1 
Slight symptoms. 
1312 
1.0 human serum (WWM). 2i 
years prior this man had been 
injected with 1,5P0 units anti- 
toxic horse serum. 
36 
do 
Marked symptoms. 
1313 
0.5 c. c. human serum (MJR). 3 
months prior this man had been 
injected with 10 c. c. antitetanic 
horse serum. 
i 36 
i 
1 
do 
Mild symptoms. 
1314 
1.5 c. c. human serum (MJR). 3 
months prior this man had been 
injected with 10 c. c. antitetanic 
horse serum. 
36 
1 ' 
1 
do 
Slight symptoms. 
1379 ' 
i 
10 c.c. human serum (WWM). 2i- 
years prior this man had been 
injected with 1,500 units anti- 
toxic horse serum. 
1 
1 Monkey. 
21 
0.2 c.c. normal horse (roan) 
serum into brain. 
No symptoms. 
290 
' 3.5 c. c. monkey (Billy) serum. 2 
years prior this monkey had 
1 been injected with 2 (?) c. c. nor- 
mal horse serum. 
6 c. c. normal horse (roan) 
serum intraperitoneally. 
No symptoms. 
290A 
: 3 c. c. monkey (Billy) serum. 2 
1 years prior this monkey had | 
been injected with 2 (?) c. c. nor- j 
mal horse serum. ! 
1| 
No symptoms. 
