36 
Table No. 34. — Anaphylactin in immune guinea pigs. 
G. P. 
No. 
First injection. 
Inter- 
val in 
days. 
Second injection. 
Result. 
1370A 
6 c. c. serum G. P. 1370, which 
- 2 
6 c. c. normal horse serum in- 
Mild symptoms. 
had received 10 subcutaneous 
injections 2 c. c. normal horse 
serum in a period of 17 days. 
traperitoneally. 
1373 A 
6 c. c. serum G. P. 1373, which 
2 
0.2 c. c. normal horse serum 
Mild symptoms. 
had same treatment as 1370. 
into brain. 
1369A 
6 c. c. serum G. P. 1369, which 
2 
6 c. c. normal horse serum in- 
Marked symptoms. 
had same treatment as 1370. 
traperitoneally. 
1371 A 
6 c. c. serum G. P. 1371, which 
2 
0.2 c. c. normal horse serum 
Slight symptoms. 
had same treatment as 1370. 
into brain. 
1374A 
6 c. c. serum G. P. 1374, which 
2 
6 c. c. normal horse serum in- 
Marked symptoms. 
had same treatment as 1370. 
traperitoneally. 
1372A 
6 c. c. serum G. P. 1372, which 
2 
0.2 c. c. normal horse serum 
No symptoms. 
had same treatment as 1370. 
into brain. 
G71 
0.0006 c.c. toxin A+1/153-8 c. c. 
55 
2 c. c. antitoxic horse serum 
Very severe symp- 
antitoxic horse serum (PD 
(Natl. IX) intraperitoneally. 
toms. 
09755) , 
Next day 0.2 c. c. normal horse 
No symptoms. 
(roan) serum into brain. 
30 minutes later bled for se- 
' 
rum. 
671A 
5 c. c. serum of G. P. 671T intra- 
2 
0.2 c. c. normal horse (Frank) 
No symptoms. 
peritoneally. 
serum into brain. 
666 
0.0006 c. c. toxin A+ .0002 gm. 
55 
2 c. c. antitoxic horse serum 
Very severe symp- 
antitetanic serum (Tizzoni). 
(Natl. IX) intraperitoneally. 
Next day bled for serum. 
toms. 
666A 
6 c. c. serum of G. P. 666T intra- 
2 
0.2 c.c. normal horse (Frank) 
No symptoms 
peritoneally. 
serum into brain. 
The following series of guinea pigs is a more conclusive test that 
anaphylactin may be demonstrated in the blood serum of immune 
guinea pigs. 
Guinea pigs were first immunized by repeated subcutaneous injec- 
tions of 5 c. c. normal horse serum. The pigs received from 6 to 12 
such injections, amounting to 30 to 60 c. c. About eight months after 
this treatment the pigs were tested by intracerebral injections, to 
which some responded with slight symptoms. Two of them showed no 
symptoms at all. While all the pigs were not completely immunized 
by the first treatment of subcutaneous injections, they were certainly' 
rendered immune by the second treatment, given eight months later. 
Two hours after the second treatment the guinea pigs were bled, the 
blood centrifugated, the clear serum pipetted off and injected subcu- 
taneously into normal guinea pigs. These normal pigs so treated 
were tested two days later by intracerebral injections, and all of them 
showed symptoms. 
