38 
We add the following examples showing that the presence of 
anaphylactin may not always be demonstrated. In these experiments 
the blood serum was collected in one of two ways. From guinea pigs 
Nos. 1000 to 1007, inclusive, the blood was drawn from the heart, 
allowed to clot at room temperature, and the serum pipetted off next 
morning. In guinea pigs Nos. 1138 to 1147, inclusive, the blood was 
drawn from the large vessels of the neck, defibrinated by whipping, 
centrifugated, and the clear supernatant serum used to inject into the 
normal guinea pig. 
Perhaps the long interval may account for the negative results. 
Table No. 36. — Ana'phylactin. 
G. P. 
No. 
First injection. 
Inter- 
val in 
days. 
Second injection. 
Result. 
1000 
4 c. c. normal guinea pig serum 
26 
6 c. c. normal horse (roan) se- 
No symptoms. 
subcutaneously. 
rum intraperitoneally. 
1001 
1.5 c. c. serum G. P. 8038, which 
26 
do 
No symptoms. 
had been subcutaneously inocu- 
lated 49 days prior with 0.24 c. c. 
toxine No.9+ 1/540 c. c. antitoxic 
horse serum (NY 13C) . 
1002 
2 c.c. serum G. P. 8048, which had 
26 
do 
No sj^mptoms. 
been subcutaneously inoculated 
49 days prior with 0.24 c. c. to.x- 
ine No. 9+1/360 c. c. antitoxic 
horse serum (Welc.). 
1003 
1004 
3.5 c. c. same serum 
26 
do 
No symptoms. 
No symptoms. 
4 c. c. serum G. P. 8051, which had 
been subcutaneously inoculated 
26 
do 
49 days prior with 0.24 c. c. tox- 
ineNo.9 + 1/400 antitoxic horse 
serum (Welc.). 
1005 
4 c. c. serum G. P. 8045, which had 
26 
do 
No symptoms. 
been subcutaneously inoculated 
49 days prior with 0.24 c. c. tox- 
ine No. 9 + 1/560 c. c. antitoxic 
horse serum (Welc.). 
1006 
1007 
4 c c.same serum . . 
26 
do 
No symptoms. 
No symptoms. 
6 c. c. serum G. P. 8050, which had 
been subcutaneously inoculated 
26 
do 
49 days prior with 0.24 c. c. tox- 
ine No. 9 + 1/320 c. c. antitoxic 
horse serum (Welc.), 
i 
1 
1138 
1.5 c. c. serum G. P. 8128, which 
16 
0.25 c. c. normal horse (roan) 
No symptoms. 
1 had received 27 subcutaneous 
injections normal horse serum 
i in a period of 65 days and bled 
21 days after last injection. 
serum into brain. 
1139 
3.5 c. c. same serum ... 
16 
5 c. c. normal horse serum in- 
No symptoms. 
traperitoneally. 
1140 
1.5 c. c. serum G. P. 8127, which 
19 
0.25 c. e. normal horse serum 
Mild symptoms. 
had received 27 subcutaneous 
injections normal horse serum 
in a period of 65 days and bled 
21 days after last injection. 
into brain. 
