43 
paraffin oven for one-half to one hour, washed quickly in water, and 
mounted in glycerin or dehydrated over night in alcohol and mounted 
in balsam. 
As controls in this work, tissue was used from normal pigs, from a 
pig dead of puerperal sepsis vdth marked fatty changes in liver and 
kidney, and from pigs killed with, diphtheria toxine. Sati’s method 
of osmization was also used with some of the specimens. 
Fresh and formalinized tissue was stained by the admirable method 
of Torrain Smith (nile blue sulphate) ; also, Sudan III. 
Results . — In sections stained for general study the sole difference 
from normal tissue consists in the marked dilatation of the veins and 
capillaries, especially of the stomach and intestines, accompanied by 
extravasation of blood at points where the vessels are ruptured. The 
thin-walled veins of the mucosa of the stomach are often greatty dis- 
tended. In the few instances where a ruptured point in the vessel was 
seen in section the extravasation of blood Acas from the portion of the 
A^ein nearest the inner surface of the mucosa. The A^eins of the sub- 
mucosa participated in the dilatation, but AA^ere not so markedly 
enlarged as the A^eins and capillaries of the mucosa. No evidence of 
general ghdng AA^ay of the capillaries AAuth extravasation of blood into 
the tissues AA^as noted. 
The small veins and capillaries of the intestines, kidney, heart, and 
muscles AA^ere found distended, but not nearly to the extent found 
in the stomach AA^all. No hemorrhages AA’ere obserA^ed in the heart 
AA^alls or in the liA^er and striped muscles. 
As regards fatty changes in the lining endothelium of the blood 
vessels, in the gastric mucosa or striped muscles, none AA^as obserA^ed, 
although carefully sought for. The focal fatty changes described by 
Gay and Southard AA^ere not found. Neither AA’ere the ‘‘ nodal” 
changes in peripheral neix^es made out. 
We find, then, that congestion and sometimes hemorrhage take place 
in guinea pigs dead of anaphylaxis, but these lesions are not specific. 
We AA'ere unable to demonstrate the fatty lesions and knoAA', 
further, that they occur in other states. We are therefore unable to 
confirm the obserA^ations of Gay and Southard along these lines, and 
beheve that these changes do not explain the mechanism of anaphy- 
laxis. 
