47 
The serum, i. e., another package of the same laboratory number, 
used in this case was tested for toxicity upon the following series of 
guinea pigs: 
Table No. 39. — Toxicity of serum. No. 2295. 
G. P. 
No. 
First injection. 
Inter- 
val in 
days. 
Second injection. 
P.esult. 
9135 
0.23 c. c. toxine No. 7+1/370 c. c. 
antitoxic horse serum (Natl. 
V.24). 
97 
0.1 c. c. antitoxic horse serum 
(2295) into brain. 
Dead in 20 minutes. 
9133 
0.23 c. c. toxine No. 7+1/370 c. e. 
antitoxic, horse serum (Natl. 
V.24). 
97 
0.05 c. c. antitoxic horse serum 
(2295) into brain. 
Dead in 22 minutes. 
Dead iu 11 minutes. 
9132 
0.23 c. c. toxine No. 7+1/310 c. c. 
antitoxic horse serum (Natl. 
V.24). 
97 
do 
9373 i 
1 
111 
0.23 c. c. toxine No. 7+ 1/1080 c. e. 
antitoxic horse sennn (NY. 
300) . 
97 
do 
Severe symptoms. 
9171 
0.23 c. c. toxine No. 7+1/410 c. c. 
antitoxic horse serum (Mill. 
2438) . 
07 
1 c. c. antitoxic horse serum 
(2295) subcutaneously. 
Dead in 90 minutes. 
9104 
0.23 c. c. toxine No. 7+1/400 c. c. 
antitoxic horse serum (Mem. 
C23). 
07 
1 c. c. antitoxic horse serum 
(2295) intraperitoneally. 
Very severe symp- 
toms. 
It has interested us ver}^ much to find that these two cases, and also 
others that have come to our notice, were in asthmatics. In our first 
publication we suggested that the essential lesion of serum anaphy- 
laxis is probably localized in the respiratory center, and the association 
of asthma and hypersusceptibility to horse serum in man would seem 
to lend weight to this hypothesis. The knowledge of the fact that the 
injection of horse serum into some asthmatics may be attended with 
danger should be considered in the use of antitoxin. 
In order to determine the comparative toxicity of the above two 
serums (Nos. 2277 and 2295); we submit the following experiments 
showing the toxicity of serums which have been largely used in human 
therapy without untoward effects. 
The following serums (Nos. 2364, 2369, and 2442) were kindly sent 
us b}^ Dr. A. P. Hitchens, who states ^^he has had no report whatever 
concerning an}^ untoward effect resulting from the use of either of 
these numbers:” 
No. 2364 is antitoxic serum. 
No. 2369 is antitoxic globulin. 
No. 2442 is antitoxic serum. 
