10 
Those that died were carefully autopsied, and if any lesions sug- 
gestive of tuberculosis was found an emulsion of the suspicious 
material was injected into another guinea pig. All animals alive at 
the end of two months were killed and autopsied. 
The other half of the citrated blood was placed in the ice chest and 
at the end of twenty-four hours exactly the same procedure was 
followed with it as detailed above for the centrifuged portion. 
The blood from the experimentally infected guinea pigs and rabbits 
was taken directly from the exposed heart after the animal had been 
etherized and, except in a few instances, treated as that from the human 
cases, though as a rule fermentation tube cultures were not made. 
All the smears were first dried by moderate heat, then the blood 
was laked in distilled water, air dried, fixed in the Bunsen flame, 
stained with hot carbol-fuchsin, decolorized in 20 per cent sulphuric 
acid, washed in alcohol, count erst ainecl with Loeffler blue, and 
examined for acid-fast bacilli. 
I wish to acknowledge my indebtedness to Dr. W. H. Frost, who 
kindly collected most of the specimens of blood from the cases of 
human tuberculosis, and to Doctor Smith, resident physician of the 
district tuberculosis hospital, and his assistants, for courtesies 
extended Doctor Frost in the collection of the blood from the cases 
of human tuberculosis. 
The results will be seen from the following protocols: 
Blood from cases of human tuberculosis. 
Case No. 
History of case from which 
blood was taken. 
i 
*3 O 
Acid-fast bacilli in sedi- 
ment smears. 
Growth tubercle bacilli on 
glycerin potato in 6 weeks. 
Growth in fermentation 
tube in 72 hours. 
1 
§*.“ 
H ft 
"c 
— O 
— ; 
2 5; 
S> >> 
m ~ 
< 
Result. 
— o 
pj'X 
C in 
n P 
3 c 
'X. 
X P 
< 
How blood 
was 
treated. 
[3 
z 
m 
U 5 
Death, in days later. 
Killed, days later. 
Autopsy 
findings. 
c. c. 
c. c. 
1 
Pulmonary tuberculosis, 2 
20 
15 c. c. cen- 
— 
— 
5 
i 
70 
No evidence 
R. B. 
months’ duration; slight 
trifuged. 
of tubercu- 
infiltration left apex; 
losis. 
slight cough; very slight 
5 c. c. in 
a+ 
— 
2.5 
2 
27 
Do. 
fever; bacilli in sputum. 
ice chest 
24 hours. 
2 
Pulmonary tuberculosis, 2 
20 
15 c. c. cen- 

b + 
5 
3 
70 
Do. 
G. S. 
years’ duration; infiltra- 
trifuged. 
tion at apex; temperature 
5 c. c. in 
— 
— 
— 
2.5 
4 
72 
Do. 
nearly normal; improv- 
ice chest 
ing; bacilli in sputum. 
24 hours. 
3 
Pulmonary tuberculosis; 1 
20 
15 c. c. cen- 
_ 

5 
5 
70 
Do. 
L. T. 
year duration: moderate 
trifuged. 
involvement 1 lung: im- 
5 c. c. in 
— 
— 
— 
2.5 
6 
72 
Do. 
proving; bacilli in spu- 
ice chest 
turn. 
24 hours. 
4 
Pulmonary tuberculosis; 6 
12 
7 c. c. cen- 

c + 
3 
6x 
69 
Do. 
A. W. 
months’ duration: infil- 
-trifuged. 
tra tion 1 apex; stationary; 
5 c. c. in 
— 
— 
— 
2.5 
7 
72 
Do.' 
bacilli in sputum. 
ice chest. 
a Many dumps of 3 to 30 acid-fast bacilli in smears. 
b Hay bacillus. 
c Coccus. 
