15 
t 
BLOOD FROM RABBITS EXPERIMENTALLY INFECTED. 
All the following rabbits from which blood was taken were inocu- 
lated subcutaneously with 0.5 c. c. of an emulsion of a glycerin 
potato growth of bovine tubercle bacilli. The rabbits were etherized 
at varying intervals after infection, the heart exposed and the blood 
drawn directly from the heart into a sterile syringe. In most in- 
stances a guinea pig was given 5 c. c. of the rabbit’s heart blood 
intraperitoneally. 
About 10 c. c. of the blood was added to sterile citrate of soda 
solution and placed in the ice chest overnight. Cultures were made 
on glycerin potato both from the whole heart’s blood and from the 
sediment of the citrated blood. With a few exceptions two smears 
were made from the sediment of the citrated blood alone. After 
the blood was drawn from the heart the rabbit was killed and a 
careful autopsy made to determine if the animal was tuberculous. 
The details will be seen from the following protocols: 
Rabbit No. 1 . — Blood drawn fourteen days after infection. No 
naked-eye evidence of tuberculosis at autopsy. No cultures made 
from the blood. 
Five c. c. whole heart’s blood injected into guinea pig No. 1A, 
weight 310 grams; killed seventy days later, weight 415 grams; 
generalized tuberculosis. 
Rabbit No. 2 . — Blood drawn twenty-one days after infection. 
No naked-eye evidence of tuberculosis at autopsy. 
Five c. c. whole heart’s blood injected into guinea pig No. 2A, 
weight 290 grams; died two days later; no evidence of tuberculosis at 
autopsy. Glycerin potato culture of whole blood negative. 
Ten c. c. blood in citrate soda solution in ice chest twenty-four 
hours. No tubercle bacilli found in sediment smears; no growth 
on glycerin potato in six weeks; 2.5 c. c. sediment intraperitoneally 
in guinea pig No. 2B, weight 285 grams; killed 64 days later, weight 
350 grams; no evidence of tuberculosis at autopsy. 
Rabbit No. 3 . — Blood drawn twenty-seven days after infection. 
General tuberculosis at autopsy. 
Whole heart’s blood on glycerin potato gave growth of tubercle 
bacilli. Six c. c. whole heart’s blood injected into guinea pig No. 3 A, 
weight 300 grams; killed fifty-eight days later, weight 490 grams; 
general tuberculosis at autopsy. 
Fourteen c. c. blood in citrate soda solution in ice chest twenty-four 
hours. No tubercle bacilli in sediment smears. Growth of tubercle 
bacilli from sediment on glycerin potato. Two and five-tenths c. c. 
sediment intraperitoneally into guinea pig No. 3B, weight 310 grams; 
died fifty-seven days later, weight 365 grams; general tuberculosis 
at autopsy. 
