17 
culosis at autopsy. Growth of tubercle bacilli from sediment on 
glycerin potato. 
Rabbit No. 8. — Blood drawn twenty-seven days after infection. 
General tuberculosis at autopsy. 
Whole heart’s blood on glycerin potato gave no growth of tubercle 
bacilli. Five c. c. whole heart’s blood into guinea pig No. 8A, weight 
300 grams; killed fifty-nine days later, weight 415 grams; general 
tuberculosis at autopsy. 
Ten c. c. blood in citrate soda solution in ice chest twenty-four 
hours. No tubercle bacilli in sediment smears. Four c. c. sediment 
intraperitoneally in guinea pig No. 8B, weight 300 grams; died fifty- 
seven days later, weight 420 grams; general tuberculosis at autopsy. 
No growth of tubercle bacilli from sediment on glycerin potato. 
BLOOD FROM GUINEA PIGS EXPERIMENTALLY INFECTED. 
All the following guinea pigs from which blood was taken were 
inoculated subcutaneously with five-tenths c. c. of an emulsion of 
a glycerin potato growth of bovine tubercle bacilli. The guinea 
pigs were etherized at varying intervals after infection, the heart 
exposed and the blood drawn directly from the heart into a sterile 
syringe with citrate of soda solution. Usually about five c. c. of 
the whole citrated blood was at once injected into the peritoneal 
cavity of a fresh guinea pig. 
The remainder of the blood in the syringe was placed in the ice 
chest over night. 
Cultures were made on glycerin potato both from the whole heart’s 
blood and from the sediment of the citrated blood. With a few 
exceptions two smears were made from the sediment of the citrated 
blood alone. After the amount of blood desired had been taken 
from the heart the guinea pig was killed and a careful autopsy made 
to determine if the animal was infected with tuberculosis. The 
details will be seen from the following protocols: 
Guinea pig No. 100. — Blood drawn fourteen days after infection. 
General tuberculosis at autopsy. No cultures made from the blood. 
Five c. c. whole heart’s blood injected into guinea pig No. 100A, 
weight 305 grams; killed sixty-seven da}^s later, weight 450 grams; 
no evidence of tuberculosis at autopsy. 
Guinea pig No. 101. — Blood drawn fourteen days after infection. 
General tuberculosis at autopsy. No cultures made from the blood. 
Four c. c. whole heart’s blood into guinea pig No. 101 A, weight 
340 grams; killed sixty-seven days later, weight 540 grams; no evi- 
dence of tuberculosis at autopsy. 
Guinea pig No. 102. — Blood drawn sixteen days after infection. 
General tuberculosis at autopsy. 
7987— Bull. 57—09 2 
