The Somatic Nuclei of Certain Cestodes. 
149 
all definite outlines and appearing as darker staining indefinite blotches 
in the tissue. 
3. Treatment with 1% potassium hydrate for periods of 24, 40 and 
60 hours respectively. After the 24 hour period, the material was teased 
and stained in haematoxylin, carmine, and methylen blue. Material after 
treatment for 40 and 60 hour periods was fixed in Solutions A and G 1 ) 
respectively, and later sectioned, and stained in VI. In this material 
nuclear outlines were either obliterated or rendered indistinct. Most of 
the staining matter in botli typical and atypical nuclei was dissolved, 
only a few indistinct granules persisting. This dissolution was evidenced 
by the refusal of the cells to take the nuclear stain. The cell bodies 
themselves were not attacked. This renders it probable that the stain- 
ing matter of the atypical nuclei is deposited in the cytoplasmic stroma, 
rather than being a direct modification of the latter. 
4. Treatment with 1% hydrochloric acid. The methods employed 
were identical with those given under (3), but the results showed both 
typical and atypical nuclei unaltered, neither »nucleoli «, membranes or 
graniüar matter having been attacked. 
5. Similar treatment with 50% hydrochloric acid 2 ). The staining 
matter of both typical and atypical nuclei was partly or wholly dissolved. 
6. Artificial digestion in a solution composed of one fifth gram of 
pepsin in 200 cc. of y 4 % hydrochloric acid for the above mentioned 
periods, and subsequent treatment as above, except that 40 as well as 
64 hour material was fixed in solution F. Neither typical or atypical nuclei 
were affected. In sections from material treated for 40 hours I find the 
general cytoplasm apparently digested, but the nuclei not attacked. 
7. Artificial digestion in a solution composed of one gram of pan- 
creatin in 200 cc. of a 1% sodium carbonate solution, and subsequent 
treatment as in (6), except that methyl green was substituted by mistake 
for light green in counter-staining the 40 hour material. After the 24 hour 
treatment the results were similar to those obtained with pepsin, but 
after 40 hours the nuclear matter was partly, and after 64 hours largely 
dissolved. 
These results show conclusively, I believe, that the staining matter 
in the atypical nuclei is similar to the »nucleoli« of the typical nuclei, 
x ) See table of fixatives and stains, pp. 140 — 141. 
2 ) After 68 hour treatment material was also teased and stained in methyl green. 
The results were similar to the others obtained by this method altho the stain had 
faded somewliat when examined. 
