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Freda M. Bachmann 
gyne which can be taken for a spermatium nucleus. The densely staining 
body in Figure 38, PI. XXXIII, is very probably the degenerating 
nucleus of the end cell of the trichogyne. The chromatin is no longer 
distributed in granules but massed so that there is no organized structure 
in the nucleus ; the nuclear membrane is still visible and a densely stain- 
ing granule appears at one end. This granule may be a centrosome. Such 
a mass of stainable material is often found in old trichogynes, and in 
each c-ase, since it is not accompanied by a normal nucleus, I have inter- 
preted it as the disintegrating nucleus of the end cell. In the terminal 
cell shown in Figures 44 — 47, PI. XXXIV, no such structure was found. 
This represents a very late stage and it may be that the disintegration 
of the nucleus is complete, or it may be that the body at a in Fig. 48 is 
the remains of this nucleus and that it has moved downward into the next 
cell. The dense staining body shown in Figure 16, PI. XXXI, may 
possibly be the remains of the end cell nucleus. 
The remaining cells of the trichogyne and ascogone now begin to 
show changes. They grow and the nuclei become much larger; each has 
a conspicuous nucleole and much chromatin in small scattered masses 
mostly on the nuclear membrane. The chromatin granules are connected 
by linin threads. The cytoplasm is becoming denser and more granulär. 
I have already noted that the cross walls of the carpogone when it is 
formed are entire. It is very evident now that they are becoming per- 
forated in the middle. Figure 18 is especially interesting in this Connec- 
tion. The first septum at a is plainly perforated, and a Strand of cyto- 
plasm extends from one cell into the other. In the penultimate cell are 
two nuclei which I am inclined to think have been produc-ed by a division 
of the primary nucleus of this cell; one of the two nuclei is evidently 
migrating into the branch of the trichogyne which has been formed at 
this place. At the next lower septum and lying partly within the opening 
in the center is a mass of chromatic material. This may be a nucleus 
which is drawn somewliat out of shape in the act of passing through 
the septum; but the preparation also suggests that there may be two 
small spindles here. It seems at least to be nuclear material and just 
about to pass through the septum. It is possible that this is a male nucleus, 
whether at rest and somewliat out of shape or in a stage of division. The 
very evident Perforation of the first septum, together with the fact that 
there are two other nuclei beside the one in question in the penultimate 
cell, makes it highly probable that this mass of nuclear material has 
migrated from the terminal cell into and through the adjoining lower 
cell and is just passing into tlie third cell. In the 12th cell (counting from 
