394 
Freda M. Bachmann 
the triple stain, these s wollen, gelatinous cross walls of the trichogyne take 
a deep orange color with even a very short exposure to Orange G. Tliis 
makes tliis part of the trichogyne extremely conspicuous and easy to 
find in the sections. The blue staining terminal cells and the brown cross 
walls are very conspicuous even after the apothecium has beeil formed. 
The nueleus in eacli of these cells very soon becomes smaller (Fig. 40, 
PI. XXXIII), and the chromatin is no longer massed in granules but 
is evenly distributed on the nuelear membrane so that the latter appears 
in section as a circle with a much thickened circnmference. It is possible 
tliat the nueleus at a in Figure 49, PI. XXXIV, is in the early stages 
of transformation to such a condition. In the same figure, the disinte- 
grating nueleus at l> resembles a normal nueleus onlv in outline. In 
Figure 48 at c the mass of stainable material at one end of the cell may 
be partly chromatin. Along with the nuelear changes in the trichogyne 
cells go changes in the cytoplasm. The cells maintain their cylindrical 
shape for some time, but bv the time the thickening of the cross walls 
is greatest and the nueleus is disappearing (Fig. 29, PI. XXXII), the 
cytoplasm has beeome denser, the plasma membrane has been drawn 
away from the side walls, and these walls begin to bend in toward the 
center of the cell giving the cell somewhat the shape of an hour-glass. 
At the cross walls the hyphae remain as wide as before. As the cyto- 
plasm becomes denser, small, irregulär, red-staining masses appear 
(Figs. 28, 29), which increase in size until much of the cell contents is 
stained deeply (Fig. 27). However, as disintegration continues still 
further, the mass of cytoplasm stains less deeply; but the brown walls 
remain conspicuous. 
The cells of the ascogone have now increased in size and the per- 
forations of the cross walls have beeome more evident. Tliis increase in 
size of the cells is not accompanied by an increase in the stainable sub- 
stance of the cytoplasm. This continues to decrease in amount until 
there is very little in the cells. The nuclei, on the other liand, maintain 
their size. The chromatin is no longer so finely distributed; in many 
nuclei it is collected in larger masses, doubtless indicating the early pro- 
phases of division. Figures 41 — 43, PI. XXXIII, represent a stage some- 
what later than tliat shown in Figure 19, PI. XXXI. Here the cells 
are still uninucleate with the exception of the one at a which has two 
nuclei. There is no evidence of a former cross wall between these two 
nuclei, whence it follows either that the two result from the division 
of a single nueleus or that one nueleus has come from another cell. The 
nuelear membranes are not distinct, but the arrangement of the chromatin 
