396 
Freda M. Bachmann 
are empty cells on eitlier side from which some of these nuclei may have 
migrated. The divisions of tlie nuclei in a group are not simultaneous 
(Fig. 50 at d). Wliile in some cases the number of nuclei in a group 
is a multiple of two, tliis is not always true. In the group of twelve above 
mentioned, even if the two larger should divide there would be only 
fourteen instead of sixteen as woiüd be expected to result from a regiüar 
series of divisions from one or two nuclei. However, it may be that part 
of the daughter nuclei of one cell migrate in one direction and part in 
another direction. From the size of the nuclei no conclusion can be 
drawn as to their origin. At e in Figure 50 are two very small nuclei, 
with the remains of the spindle still between tliem. It might be sug- 
gested that these are male nuclei, but in Figure 19, PI. XXXI at b, 
the smaller of the two nuclei, which I think is probably a male nucleus, 
is much larger than the nuclei in the spermatia; and from Figure 41, 
PL XXXIII at ct, it seems very probable that this inc-rease in size of the 
male nucleus within the cells of the ascogone is continued until it equals 
in size eac-h female nucleus. Because of the perforations in the cross 
walls of the ascogone, and of such nuclear behavior as I have shown in 
Figures 18 and 19, PI. XXXI, it can scarcely be doubted that there 
is a migration of male nuclei into the ascogone, whether or not a fusion 
occurs later. Some of the ascogone nuclei are very slow to divide. 
For example, in the cell shown in Figure 50, PI. XXXIV at c, the 
primary nucleus has probably not divided at all. If there is a migration 
of a spermatium nucleus or of a nucleus resulting from the division of 
the spermatium nucleus into eaeh cell of the ascogone, it may be that the 
presence of this second nucleus is the Stimulus which causes the primary 
nucleus of that cell to divide; in such cases, the failure of the ascogone 
nucleus to divide may be due to the absence of a spermatium nucleus. 
It may be too that a nuclear fusion must precede the division of the 
ascogone nuclei. 
There are about five, perliaps six, chromosomes on the spindle of 
these smaller ascogone nuclei (Fig. 50, PI. XXXIV). This is the same 
chromosome number that is found in the nuclear divisions in the vege- 
tative hypliae, and is not what would be expected if there had been a 
fusion of ascogone nuclei in pairs. However, it is not impossible that 
such a fusion has occurred and that the chromosomes on these spindles 
are bivalent, but such a condition would be out of harmony with all 
known cases. It might also be that if there is a fusion of nuclei in 
the ascogone, this does not take place until the nuclei have divided 
several times. 
