400 
Freda M. Bachmann 
fusions, one certainly in tlic ascus, tke otlier very probably in the 
ascogone. 
I have no preparations showing the spindle of the second division. 
In the third division there are probably six chromosomes. In Figure 76, 
wliieh shows this division, there are at least five chromosomes. The 
figure suggests six, but this is not so evident when studying the pre- 
paration, and besides one of the six may be a nucleole. In the third 
division therefore, the gametophytic number again appears. Hence there 
must be a second reduction in number either in the second or in the third 
division of the ascus. In the spindle of the third division the central 
bodies are very conspicuous and in sorne cases at least, they are plainly 
double (Fig. 76). I have not succeeded in staining the aster if one is 
present in the metaphase stage of this division. 
After the third division is completed each nucleus is drawn out into 
a long beak witli the central body in contact with the end of the beak 
and also with the plasma membrane of the ascus. The plasma mem- 
brane is sometimes pulled in somewhat toward the nucleus. The rays 
of the aster, which is now very evident, are bent backward and around 
the nucleus to form the spore membrane (Fig. 77), in the manner first 
desc-ribed by Harper (50) for several ascomycetes. The cytoplasm of 
the ascus is at all times very finely granulär with an occasional larger 
and more conspicuous granule. Tliere are never any large number of 
“metachromatic bodies” in the cytoplasm of the ascus such as are com- 
monly found in the asci of many other forrns. 
The uninucleate spore enlarges and elongates to about twice its 
width, and the eiglit spores eompletely fill the ascus before further divi- 
sion occurs. With the growth of the spore the epiplasm disappears so 
tliat finally none is left and the ascus is filled by the eight large spores. 
The nucleus enlarges considerablv. The chromatin becomes distributed 
as in a resting nucleus and a large nucleole is formed, even before the 
spore is eompletely delimited (Fig. 77). The cytoplasm of the spore is 
always spongy ratlier than granulär (Fig. 78). The mature spore is rnuri- 
form (Fig. 83). The nuclear divisions show a conspicuous large central 
body at either pole and five or six chromosomes. My preparations fail 
to show spindle fibers. After each division cross walls are formed. 
The first three or oceasionally the first five septa divide the spore 
transversely (Figs. 79, 80). Then there are formed two longitudinal 
walls at right angles to each other (Fig. 82) which divide all except 
the end cells of the spore (Fig. 83). This results in a ten- or cight- 
een-celled spore. 
