Nuclear Division in Funkia. 
ßy 
M. G. Sykes. 
Girton College, Cambridge, & Batlinrst Student of Xewnham College, Cambridge. 
With plates VIII— IX and 1 figure in tke text. 
The material necessarv for an investigation of tlie reduction di- 
vision in the pollen mother cells of Funkia ovata and Funkia sie- 
boldiana was collected for me before the results of Strasburger’s 
and Miyake’s * 2 ) recent papers dealing with the subject were known. 
After some consideration, it appeared to me that there still remained 
several points of interest which would repay further study. 
My thanks are dne to Mr. R. P. Gregory both for collecting 
and preserving the material used in this research and also for his 
kind interest and help in my work. 
I. Methods. 
The material, consisting of flower buds of F. ovata and F. sie- 
boldiana , was fixed in chromacetic and dehydrated by evaporation 
of dilute glycerine; from the strong glycerine it was transferred to 
absolute alcohol and embedded in paraffin by means of the cedar 
wood oil metliod. For staining, Heidenhain’s iron alum-haematoxy- 
lin was usually employed, though it was often found advisable to 
compare the preparations thus obtained with others which had beeu 
treated with Flemming’s triple stain. 
The different series varied in thickness, but as a rule sections 
from 3—5 u thick were found most useful for the study of the 
b E. Strasburger; P. J. 1906. p. 1. 
2) K. Mn-AEE. P. J. 1906. p. 84. 
