Amitosis in the Ovary of Protenor belfragei and a Study of the Chromatin Nucleolus. 191 
the egg was dropped front a height that caused it to rebound from 
the bottom of the cage. These observations were confirmed in the 
field, where in three cases the insect was observed to drop her egg 
from the top of a blade of grass two feet high allowing it to fall to 
the sod below. 
In the laboratory we isolated matirre females, some of them while 
mating, keeping a record of the number of eggs they subsequently depo- 
sited, in order to determine approximately the number deposited by 
each individual daily diuing a definite period. In this experiment we 
isolated fourteen individuals and the records sliow that in the majority 
of cases an individual deposits from 1 to 3 eggs within twentyfour hours, 
though in two cases 8 eggs were deposited in one dav, in two cases 6 eggs, 
and in three cases 5 eggs. Within a period of six days one individual 
deposited 23 eggs, another 18 eggs, two deposited 15 eggs and one 13 eggs. 
The other individuals were below this average. 
Eggs laid in the summer and fallen do not hatch out until the following 
spring. We obtained our first catch of these young bugs in May 1910. 
The very young larval stages were found and identified by Mr. Bueno, 
and from that date we were able to secure successive stages in their deve- 
lopment from probably the 2nd moidt until maturity. 
Ovaries of insects have been studied for nearly a Century and at 
intervals they have given rise to much discussion as to the origin of 
their various cell elements and the relation of these elements to the germ 
cells. A brief historical sketch of the observations and conclusions of 
the investigators of the insect ovary is given in Hennegüy’s very val- 
uable monograph, “Les Insectes 1 '. In 1886 Korschelt published a 
detailed account of the results of his own investigation of a number 
of forms and compared his conclusions with those of earlier investigators, 
his work being directed principaUy to determining the relation existing 
between the nourishing cells, the epithelial cells and the germ cells. His 
results presented evidence in Support of his criticism of Will’s conclusion 
as to the relation these elements hold to one another. 
Will (1885) drew his conclusions from the study of two Hemiptera, 
Notonecta and Nepa. Korschelt also investigated these same forms, 
and we shall at first limit our comparison of Korsciielt's results to his 
observations on these two Hemiptera and determine if possible, what 
facts observed by both investigators agree with those we shall try to 
demonstrate in Protenor. 
Before attempting a comparison of the cytological details, we will 
briefly describe that portion of the ovary of Protenor in which we find 
