Amitosis in the Ovary of Protenor belfragei and a Study of the Chromatin Nucleolus. 223 
of chromosomes is not more than half the normal somatic number which 
is 32 in both cases. . . . The great frequency with which this division 
occurs suggests stronglv that there are several generations of cells show- 
ing the reduced number of chromosomes.” 
These observations as to the presence of pseudo-reduction in cells 
outside the maturation divisions are supported in Protenor. Pseudo- 
reduced groups of chromosomes are found in cells of the terminal chamber 
— not only in zone C but in zones B and A — and these facts are in 
accord with Buchner’s observations on Gryllus. He finds tetrads in 
the oögonial divisions of this form. 
A small group of reduced chromosomes is shown in photo 35, plateXVI 
and a comparison of this group with the earlier stages of the gemiinal 
vesicles of plate XVIII, photos 62, 63 etc., makes it exceedingly doubtful 
whetlier such a reduced group is to be interpreted as an early stage of 
development of the germinal vesicle — especially as such groups are 
found in zone A as well as in zone C. 
Photo 12, plate XV demonstrates a case of pseudo-reduction in one 
of the large nuclei of zone B. Such nuclei cannot possibly be confounded 
with the germinal vesicles for we have never found germinal vesicles 
of the stage shown in photos 83 — 88, plate XX in the terminal chambers. 
We have found them only in the eggs we have taken from the egg chambers. 
Prophases of the large nuclei of zone B are shown in photos 9 — 11 and they 
in no way resemble the germinal vesicle prophases shown in the photos 
of plates XIV and XV. It would seem that the evidence in Protenor 
Supports the claim that pseudo-reduction at least in some forms is not 
confined to the maturation divisions, this indicating that variability is 
not limited to the morphology of the chi’omosomes but may be extended 
to their behavior also. 
We believe that in Anasa tristis we demonstrated facts which it 
must be conceded indicate at least a marked variability in the behavior 
of the accessory chromosome of this form. We demonstrated by a full 
series of photographs that the accessory chromosome can divide in the 
second maturation spindle, a phenomenon which normally ought not to 
occur if the theories surrounding this chromosome have a foundation 
in fact. 
We would like to correct the impression sometimes given that we 
intended to deny the observations of those who claim that the accessory 
chromosome may fall to divide in the second spindle of Anasa tristis. 
We never for a moment doubted that the accessory chromosome fre- 
quently fails to divide in the second spindle, though the fact that we 
